Human C1q-Ab (Anti-Complement 1q Antibody) ELISA Kit

Human C1q-Ab (Anti-Complement 1q Antibody) ELISA KitThe Human C1q Ab (Anti Complement 1q Antibody) ELISA Kit measures C1q Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Human C1q-Ab (Anti-Complement 1q Antibody) ELISA Kit measures C1q-Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antigen. Then, it binds with C1q-Ab bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of C1q-Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.