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Description
Rat AQP-2 (Aquaporin 2) ELISA KitThe Rat (AQP 2) Aquaporin 2 ELISA Kit measures Aquaporin 2 in Rat samples. The plate has been pre coated with Rat AQP2 antibody. AQP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat AQP2 Antibody is added and binds to AQP2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated AQP2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
The Rat (AQP-2) Aquaporin 2 ELISA Kit measures Aquaporin 2 in Rat samples. The plate has been pre-coated with Rat AQP2 antibody. AQP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat AQP2 Antibody is added and binds to AQP2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AQP2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat AQP2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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