SKU: 46627925433

Human IER5 (Immediate Early Response Gene 5 Protein) ELISA Kit

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Description

Human IER5 (Immediate Early Response Gene 5 Protein) ELISA KitThe Human (IER5) Immediate Early Response Gene 5 Protein ELISA Kit measures Immediate Early Response Gene 5 Protein in samples. The plate has been pre coated with Human IER5 antibody. IER5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IER5 Antibody is added and binds to IER5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IER5 antibody. After incubation unbound

The Human (IER5) Immediate Early Response Gene 5 Protein ELISA Kit measures Immediate Early Response Gene 5 Protein in samples. The plate has been pre-coated with Human IER5 antibody. IER5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IER5 Antibody is added and binds to IER5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IER5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IER5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

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SKU: 46627925433

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