SKU: 47352594356

Rat VIP (Vasoactive Intestinal Peptide) QuickTest ELISA Kit

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Description

Rat VIP (Vasoactive Intestinal Peptide) QuickTest ELISA KitThe Rat VIP (Vasoactive Intestinal Peptide) QuickTest ELISA Kit measures VIP in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples

The Rat VIP (Vasoactive Intestinal Peptide) QuickTest ELISA Kit measures VIP in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains VIP, a capture antibody-VIP-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of VIP in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 47352594356

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LLB
Waukegan, US
★★★★★ 5
Wow!
Format: Kindle
I picked 5 stars because this was the book I've been searching for! I have been on a quest for enlightenment and spiritual growth for years. And needed a way to wash away the heavy feelings and the "stuck" I was feeling. And wanted to know why the same events kept happening to me. And in just a few pages I was instructed on how to reach the records with ease. I have been in the records a few times now. And have been amazed at what I learned about myself. I thought seeing the patterns would be hard. But it's not. All the information in that moment is right there. And as you unwind the karma allowing, surrender and begin to heal instantly other events that you had the same pattern happening shows up too. And even deeper healing happens. I am now well on my way. After just a few trips to the records I can already feel the positive changes happening. I no longer feel that heavy stuck feeling. I have finally found the peace, love and joy I've been missing in my life. And I think everyone should learn how to do this. For this world and the billions of people who live here needs more love now more than ever. Thank you Melissa for writing a book so easy to use and understand. Love and Light, Peace and Love to all.
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Reviewed in the United States on October 30, 2018
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Terry Vartanyan
Alexandria, US
★★★★★ 5
Both fascinating and practical for our lives
Format: Kindle
Not only was this book informative and a real thrill to read, I feel driven to heal what I can in this life, and then also ask for gifts that may have been unreachable due to fear and other blocks. Grateful to have found this book, a real gift to me!
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Reviewed in the United States on May 1, 2025
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Brian Halley
Natrona Heights, US
★★★★★ 5
Wow, informative must for career path
Format: Paperback, Format: Paperback
Great resource for any student, young or old. Solid advice and support continues with bonuses after you read with links and resources. Even some one on one with the author, worth much more than the initial cost of the book. Very informative and uplifting. The author wants to make your career choice path easier. A must have for college bound students. It’s like finding a treasure map to all the right ideas! Front to back a super read. I purchased the physical copy and plan to share it with a high school student.
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Mark
Charlottesville, US
★★★★★ 5
Great food for thought!
Format: Kindle
What a great resource for young people tackling the seemingly impossible task of figuring out what they want to do with their lives. Facing this challenge can be overwhelming, but this book offers practical advice and insight to navigate these uncharted waters.
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Darrin
Los Angeles, US
★★★★★ 3
Incomplete
Format: Paperback
Incomplete- overall has some good content, but missing networking, informational interviews.
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Reviewed in the United States on June 3, 2026

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