Human BMG/Beta2-MG (Beta-2-Microglobulin) ELISA Kit

Human BMG/Beta2-MG (Beta-2-Microglobulin) ELISA KitThe Human BMG Beta2 MG (Beta 2 Microglobulin) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human BMG 2 MG. During the reaction, Human BMG 2 MG in the sample or standard competes with a fixed amount of Human BMG 2 MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BMG 2 MG. Excess conjugate and unbound sample or standard are washed away, and

The Human BMG/Beta2-MG (Beta-2-Microglobulin) ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human BMG/β2-MG. During the reaction, Human BMG/β2-MG in the sample or standard competes with a fixed amount of Human BMG/β2-MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BMG/β2-MG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human BMG/β2-MG in tested samples can be calculated by comparing the OD of the samples to the standard curve.