SKU: 65646835691

Bovine PGF2ALPHA (Prostaglandin F2Alpha) ELISA Kit

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Description

Bovine PGF2ALPHA (Prostaglandin F2Alpha) ELISA KitThe Bovine PGF2ALPHA (Prostaglandin F2Alpha) ELISA Kit measures PGF2Alpha in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with PGF2. PGF2 in the sample or standard competes with a fixed amount of biotin labeled PGF2 for sites on a pre coated antibody specific to PGF2. Free components are

The Bovine PGF2ALPHA (Prostaglandin F2Alpha) ELISA Kit measures PGF2Alpha in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with PGF2α. PGF2α in the sample or standard competes with a fixed amount of biotin-labeled PGF2α for sites on a pre-coated antibody specific to PGF2α. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of PGF2α in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 65646835691

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4.1 ★★★★★
Based on 26 reviews
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J
Verified Purchase
Jacob
Belleville, US
★★★★★ 4
Dog approved!
Flavor Name: Chicken, Size: 12 Ounce (Pack of 1)
My dog, a half pit bull and half chocolate lab, has a very sensitive stomach. With that in mind I have to always be wary of the things I buy her. With that in mind these seemed to work great! They don’t last as long as normal raw hide, so there is less “chew time” but she was excited when she received them! If your looking for a quick treat or reward for your dog these are great! If your looking for a bone/chewing alternative then these don’t quite meet that requirement.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 29, 2018
R
Verified Purchase
Rachierach
West Palm Beach, US
★★★★★ 5
Doberman Approved
Flavor Name: Chicken, Size: 12 Ounce (Pack of 1)
My 106lb Doberman refuses to eat anything but these and duck hearts- go figure- he does not like any type of carb, his diet consists of Taste of the Wild and boiled chicken, he gets one of these every night and wiggles his cute little butt off when he hears me getting the bag, he can hardly contain his excitement. He has NEVER had any issues of chocking or vomiting with these, I am a huge believer in sharing experiences when it comes to our fur babies, mine loves his biscuit (that's what we call it" I know it isn't a biscuit.
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Reviewed in the United States on September 29, 2020
D
Verified Purchase
danaz
Boise, US
★★★★★ 5
Great alternative to rawhide!
Flavor Name: Chicken, Size: 12 Ounce (Pack of 1)
We have been getting these for quite some time for our black lab. He eats rawhide too quickly and ends up regurgitating it all over the floor. So, on the advice of our vet, we tried something else. We tried these after many other products. These are gone as quickly as rawhide (with my dog, that's 10 minutes... TOPS!), but he doesn't have the digestive issues. The fact that they chew up similar to rawhide is a big plus, since I don't like it when he can't finish it in one sitting - that's just a yucky mess laying around on the floor. I highly recommend these SmartBones for anyone whose dog can't digest rawhide well.
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Reviewed in the United States on January 9, 2014
L
Verified Purchase
L.Whitehead
Massapequa, US
★★★★★ 5
Happy dog
Flavor Name: Chicken, Size: 12 Ounce (Pack of 1)
We have a big dog and don't like to give him rawhide products for many reasons. We came across SmartBones a couple of years ago and have bought them ever since. They work well for our dog. The challenge has been finding them in stores where we live and at a fair price. These worked.
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Reviewed in the United States on July 14, 2021
D
Verified Purchase
Denaye
West Palm Beach, US
★★★★★ 5
I order these a lot. They don't have rawhide ...
Flavor Name: Chicken, Size: 12 Ounce (Pack of 1)
I order these a lot. They don't have rawhide in them so that is my main reason. I have a small dog and I order a large because it then takes him about 45 mins to an hour to eat. Most of the time after he eats half I save the other half for the next day to make them last longer! He is a very active dog but these are his favorites. I also order them in small for little treats here and there (those last under 10 mins for him).
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Reviewed in the United States on February 27, 2016

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