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Ships within 48 hours · Estimated delivery Aug 13 - Aug 18
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Description
Human 4HNE (4-hydroxynonenal) ELISA KitThe Human (4HNE) 4 hydroxynonenal ELISA Kit measures 4 Hydroxynonenal in samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and
The Human (4HNE) 4-hydroxynonenal ELISA Kit measures 4-Hydroxynonenal in samples. Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of 4HNE in the sample. The concentration of 4HNE in the sample is then determined by comparing the O.D. of the samples to the standard curve.
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