SKU: 12032573010

Anti-Human IgG Donor Beads

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Description

Anti-Human IgG Donor BeadsProduct Specification Stability & Storage Store at 28C away from light; product shelf life is 12 months. Background Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence. Donor beads recognize Protein 1 (Tag1 label), while Acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2, the

Product Specification


Stability & Storage

Store at 2～8°C away from light; product shelf life is 12 months.

Background

Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence.

Donor beads recognize Protein 1 (Tag1 label), while Acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2, the distance between the beads becomes less than 200nm. Upon excitation at 680nm, the donor beads generate singlet oxygen, which diffuses to the acceptor beads. The acceptor beads then undergo a redox reaction, emitting light at 615nm. The signal intensity is directly proportional to the strength of the protein interaction.

This product features a simple operation process, requires no washing, and offers fast results with high sensitivity. It is capable of detecting weak interactions.

Components

Specification

Volume

250 μg

50 μL

5 mg

1 mL

25 mg

1 mL x 5

Protocol

[Required Reagents]

Name

Cat. No.

Anti-Human IgG Donor Beads

UA086109

Streptavidin Acceptor Beads

UA086090

Universal Buffer 1

UA086113

[Assay Procedure for Reference]

Assay Procedure

Assay Procedure 1 (37°C Rapid Assay)

Assay Procedure 2 (Room Temperature Assay)

​Step 1:

4μL hFC tag-M1 +4μL Biotin-M2+ 6μL Anti-Human IgG Donor Beads, Protect from light / Green light

4μL hFC tag-M1 +4μL Biotin-M2+ 6μL Anti-Human IgG Donor Beads, Protect from light / Green light

Incubation

​Shake and incubate at 37°C for 20 minutes, Protect from light / Green light

​Incubate at room temperature for 60 minutes, Protect from light / Green light

​Step 2:

Add 6μL Streptavidin Acceptor Beads, Protect from light / Green light

Add 6μL Streptavidin Acceptor Beads, Protect from light / Green light

Incubation

​Shake and incubate at 37°C for 10 minutes, Protect from light / Green light

​Incubate at room temperature for 30 minutes, Protect from light / Green light

Readout​

Instrument Readout

Instrument Readout

[Performance Validation]

•Sample Preparation:

Pre-dilute biotinylated human IgG (Bio-hIgG) to 15 μg/mL (100 nM) using Universal Buffer 1 as the stock solution, then perform serial dilutions according to the following scheme:

No.

Final Concentration (nM)

Volume of Universal Buffer 1 (μL)

Volume of High Conc. Addition (μL)

C12

1.0E+01

210

90 μL Stock Solution

C11

3.0E+00

210

90μL C12

C10

1.0E+00

180

90μL C11

C9

3.0E-01

210

90μL C10

C8

1.0E-01

180

90μL C9

C7

3.0E-02

210

90μL C8

C6

1.0E-02

180

90μL C7

C5

3.0E-03

210

90μL C6

C4

1.0E-03

180

90μL C5

C3

3.0E-04

210

90μL C4

C2

1.0E-04

180

90μL C3

C1

0

180

/

•Detection Reagent Preparation:

Name

Working Concentration

Diluent

Anti-Human IgG Donor Beads

25 μg/mL

Universal Buffer 1

Streptavidin Acceptor Beads

25 μg/mL

Universal Buffer 1

•37°C Incubation Mode Results:

Maximum Signal:47550

Minimum Signal:192

EC50= 0.067 nM

•Results of Room Temperature Incubation Mode:

Maximum Signal:23402

Minimum Signal:92

EC50= 0.036 nM

Guidelines

1. This experiment is light-sensitive; ensure all procedures are performed under light-protected conditions. It is recommended to conduct preparation, sample loading, and incubation steps under green light (illuminance below 100 LUX). 2. This product is compatible with multifunctional microplate readers equipped with Alpha detection modules. 3. Vortex thoroughly before use, or briefly centrifuge (2000×g, 5–10 seconds) to ensure complete sample retrieval. 4. It is advised to use the accompanying dilution buffer from our company for reagent preparation and sample dilution. If additional components are required, they can be directly added to this buffer. 5. To ensure comparability of experimental data across different batches, strictly control incubation temperature and duration. 6. Avoid generating bubbles during sample loading.
Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
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SKU: 12032573010

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4.4 ★★★★★
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A
Verified Purchase
Amazon Customer
Charlottesville, US
★★★★★ 4
Good enough
Color: Black
Comfortable chair. Easy to put together. Allen nuts aren't cut perfectly so sometimes hard to get the wrench in there. My biggest complaint, and I didn't even realize this until I sat down in it, the arm rests are not adjustable and they are a little short for my liking.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 17, 2026
D
Verified Purchase
DEVON
Draper, US
★★★★★ 5
COMFY CHAIR GOOD SUPPORT
Color: Dark Brown
So I needed an office chair because the one that I had wasn't really helping my back any. And it just didn't have enough cushion on the chair because when you want to sit for hours at your desk you want to be comfortable. So I took a chance on buying this chair. I thought the color was good and the price was right but I was still a little nervous because it said put together. So I got it right away only took 3 days. And the directions for pretty easy there were a couple of spots that I think we're out of order because you had to kind of play around with the screws going into the holes from the arms but I think if I were to get another chair like this I would probably do it my way because it's pretty self-explanatory but I followed the directions that came with the chair. And hey I'm 65 years old and I put this chair together. It's nice it's sleek and oh my God is it comfortable!!! Right where my lower back and sciatic is I get the support and it just feels good and I know that if I have to be at my desk for 3 or 4 hours I'm going to be comfortable. I highly highly recommend getting this chair. The only issue that I had was the driver did not leave it on my porch. I had ordered a Vizio TV a couple months ago and the driver brought it up to my porch. So I just had to go out and take two steps bring it into the house I really appreciated that. This driver didn't even come through the gate and there is no signs of any dogs because there aren't any. So this driver just left this giant heavy box it was about 35 lb in front of the garage where it was very visible from the road. And I had a dragon to my front door it was heavy I did not like that at all. But it wasn't damaged or anything and I do love the chair. I just don't like what the driver did.
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Reviewed in the United States on November 3, 2025
A
Verified Purchase
Aya
Houston, US
★★★★★ 5
Great sturdy chair and beautiful color
Color: White
I love this chair. I bought it for my home office. It is beautiful, sturdy and very comfortable. I'm not good at all with assembling even basic things but I would say that this was relatively pretty simple for someone like me to put together. Good price and good product.
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Reviewed in the United States on April 3, 2026
O
Verified Purchase
OKCity
Alexandria, US
★★★★★ 4
Reliable, Comfortable, and Good-Looking Chair — I Keep Coming Back to It
Color: White
This is my fourth time purchasing this Amazon Basics mid-back office chair, which says a lot about its overall value. It looks clean and modern, fits well in a home office, and is genuinely comfortable for daily use. I typically get about a year and a half out of each one, which feels reasonable given the price point and materials. Out of the box, the chair is easy to assemble and immediately comfortable. The padding is supportive, the armrests are well positioned, and the faux leather gives it a more upscale appearance than many chairs in this category. It rolls smoothly, swivels easily, and works well for long computer sessions without causing back or shoulder fatigue. That said, this is not a lifetime chair. After extended use, the cushioning and structure do begin to wear, and that’s usually when I replace it. Still, considering the comfort, appearance, and cost, it remains one of the best value office chairs I’ve found — which is why I keep buying it again. Pros: • Comfortable padding and good mid-back support • Clean, modern appearance that looks great in an office • Smooth rolling and stable base • Easy assembly • Excellent value for the price Cons: • Durability is moderate; expect replacement after about 1½ years • Not designed for long-term heavy-duty use Verdict: If you want a comfortable, attractive, and affordable office chair that performs well for daily use, this is a strong choice. It may not last forever, but its comfort and value make it easy to recommend — and easy to repurchase.
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Reviewed in the United States on January 18, 2026
D
Verified Purchase
Debbie Segrest
Bozeman, US
★★★★★ 5
Highly recommend
Color: Black
Very sturdy, the cushions make it super comfortable. The size and ease of movement are perfect. Assembly was super easy. A very quiet chair. Wonderful all around value.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 28, 2026

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