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Description
Rat IFN-γ Recombinant Rabbit mAb (S-3623)Product Specification Host Rabbit Antigen IFN Clone Number S 3623 Antibody Type Recombinant mAb Isotype IgG Application ELISA Reactivity Rt Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 20 C as supplied Dilution application dilution species ELISA 1: 5000 1: 10000 Rt
Product Specification
| Host | Rabbit |
| Antigen | IFN-γ |
| Clone Number | S-3623 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA |
| Reactivity | Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ELISA | 1:5000-1:10000 | Rt |
Background
Rat IFN-γ (interferon-gamma) is a 15–16 kDa, 136-amino-acid type II cytokine secreted chiefly by activated NK cells and Th1-type CD4⁺ T cells. It signals through a high-affinity heterodimeric receptor composed of IFN-γR1/IFN-γR2, activating the JAK–STAT1 pathway to up-regulate MHC class I and II molecules, chemokines such as CXCL9/10, and multiple antimicrobial effectors. In rats this molecule is encoded by the Ifng gene on chromosome 7 and is heavily N-glycosylated; it functions as a non-covalent antiparallel dimer with species-specific biological activity that is essential for macrophage activation, Th1 polarization, and control of intracellular pathogens.
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