Rat IL-1β High Sensitivity ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti rat il-1β Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il-1&beta in the sample; Binding to solid-phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and il-1&beta in samples; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, biotin labeled il-1β One copy of detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 0.71 pg/ml

Detection Range: 7.81-500 pg/ml

Recovery Range: 89-127%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 1 (IL-1) is a 17kDa extracellular polypeptide, which is divided into il-1α and il-1β two proteins. Il-1β is a protein precursor secreted by activated macrophages, which is secreted by caspase1 (casp1/ice)The activated form produced by hydrolysis. This cytokine is an important mediator of the inflammatory response and is involved in many cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 is an important family of proteins, which are derived from bioactive monocytes and participate in inflammatory and immune responses. Il-1β Elevated expression can produce a variety of different autoinflammatory syndromes, the most significant of which is the monogenic condition known as caps, which is due to the triggering of il-1β It is caused by mutations in the processed inflammasome receptor NLRP3.