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Description
Mouse FBLN2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge at 1000 x g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store the supernatant at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000 x g for 20 minutes. Remove the supernatant for testing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and allow it to equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a fibulin 2 (FBLN2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the fibulin 2 (FBLN2) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Fibulin 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Fibronectin 2, also known as fibrin-2, is a protein encoded by the FBLN2 gene. This gene encodes an extracellular matrix protein belonging to the fibrin family. This protein binds to various extracellular ligands and calcium. It may play a role in organ development, particularly in the differentiation of cardiac, skeletal, and neuronal structures. Alternative splicing transcript variants encoding different isoforms have been identified. FBLN2 has been shown to interact with laminin α1, laminin α5, and perlecan. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.6 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Dog Approved - Highly Preferred Over Standard Toothbrush
Color: Neon (4-pack)
These pet finger tooth brushes work well for me and my dog. He most definitely tolerates these so much more than a standard tooth brush. I believe I am able to reach more of his teeth and that I'm more effective brushing with these than I am with a standard brush. I put one on each of my index fingers. This makes it easier to do each side and the top and bottom without having to twist and turn to reach everything. The size is just right for my index fingers. They are a little tight getting them on and I have found that wetting my finger and the inside of the finger brush makes it a lot easier to put them on. The bristles on the finger brushes are soft, yet they are durable enough to give some good scrub quality and gum massage. These are a good price for what you get. I purchased the four-pack at right about $16.00. I have gotten numerous uses so far out of the two that I have been using. I will definitely purchase these again when it is time.
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Reviewed in the United States on July 13, 2025
★★★★★ 4
Probably Best for Medium-Large Size Dogs
Color: Blue (2-pack)
This finger cot–style toothbrush works well and is a simple way to brush your dog’s teeth, especially if they’re not comfortable with a traditional toothbrush. The material is soft yet effective at cleaning, and it gives you good control while brushing.
The only drawback is that it’s a little large for my miniature Dachshund’s mouth, making it somewhat difficult to reach all areas comfortably. It would probably be a better fit for medium or larger dogs. Even so, it gets the job done and is a good option for introducing dogs to regular dental care.
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Reviewed in the United States on May 31, 2026
★★★★★ 5
The easiest and best tooth brush!
Color: Pink (2-pack)
Ive tried several other dog tooth brushes for my 74 lbs Coonhound. This style and brand is the only one that is easy and works for us. It's easy to move your finger around and all sides of it have the cleaning nodules so no matter which way your finger turns, they are always touching her teeth. I like to try to open her mouth and get the inside of the mouth too. I loop my finger around her fangs. It is soft without harder parts so it doesn't make her pull away. This brush is perfect. It's a good quality and comes with a container for storage.
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Reviewed in the United States on March 3, 2026
★★★★★ 5
Total GAME CHANGER - incredibly effective toothbrush for dogs
Color: Clear (2-pack)
This silicon finger brush is incredibly easy to use - and so EFFECTIVE (more on that in the last paragraph this review). At first, it seemed a little hard to get on my finger but I simply got my finger wet first under the faucet and it slipped right on with virtually no effort. And once on, it's easy to take off but doesn't slip off accidentally when in use - so - perfect! Quick & simply to clean - rinses off in the sink in seconds, and ready to use the next time. And, my dog not only lets me clean her teeth - she actually like it (this from a dog who used to hate it when I would try to brush her teeth with a regular doggy toothbrush).
This silicon "brush" is incredibly maneuverable - way more so than standard dog toothbrushes or regular finger brushes. So much so it makes it super easy to not only get those normally impossible to reach back molars - AND - even the back sides of the teeth! With a normal dog tooth brush (or finger brush) my dog's clamping down made that impossible. Now, it's not only possible - it's easy!
Our dog's canine teeth had build-up but it was not until I pulled her gums back and looked at her back molars that I saw how bad things had gotten - and we have a young dog! Her back molars not only had build-up but some appeared to be stained a tea colored brown that did not come off with my fingernail. It was pretty disheartening and I felt terrible. How could this happen so quickly? The vet said it was plaque (and not yet tartar) and that it wasn't bad enough to warrant a professional cleaning - yet - but that I needed to do a better job brushing. He said: "I can see exactly where your brushing stops" (referring to the condition on the back molars which I had not been able to do with a regular brush as my dog just wasn't having it).
Tips: here's what's working for us. Instead of squeezing out a pea sized blog of tooth paste onto this brush, I put a tiny dab - that it's - on the very tip of the brush and focus my efforts on two of our dog's worst teeth on one side - on top and then on the bottom. Then I put another small dab and repeat on the other side of her mouth with 4 teeth on that side. Then, I use another small dab and focus work on another group of teeth. All totaled, I'm using maybe 4 to 6 very small dabs of dog toothpaste, probably half the size of pea collectively, perhaps less. That's it. However much you choose to use is up to you - be sure and use an amount that's safe and effective for your dog.
Effectiveness: this works and it works well! In just 3 days using the same vanilla flavored enzymatic toothpaste by CET I was seeing improvement. By day 7, that improvement was so obvious I was actually surprised by how much better they looked. It's now been a little over two weeks and they are looking considerably better! Conservatively, I'm thinking a 50% improvement. Not bad for just 2 weeks, right? I'm actually think I'm going to be able to completely reverse things - we'll see. I'll try and update this review again in a few weeks and let you know how it's looking but in the meantime, I am absolutely thrilled with this brush. I paid $16 and it came 2 in a pack) and the first (and only one) I've been using still looks brand new after it's rinsed off. All in all - this brush is a game changer! I can now brush our dogs back teeth and even the back sides of her teeth, all in less than a couple minutes a day.
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Reviewed in the United States on August 23, 2020
★★★★★ 3
Plastic not a brush
Color: Clear (4-pack)
Love the idea, dont care for the texture. It would be nice if they were soft brush and not plastic.
Would retuen but i missed the window.
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Reviewed in the United States on May 22, 2026