SKU: 16284814085

Mouse Pro-C3 ELISA Kit

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Description

Mouse Pro-C3 ELISA KitProduct Specification Usage Specimen Requirements 1. Specimens should be extracted as soon as possible after collection, following the relevant literature. Experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, specimens can be stored at 20C, but repeated freeze thaw cycles should be avoided. 2. Samples containing sodium nitrate (NaN3) cannot be assayed, as NaN3 inhibits horseradish

Product Specification


3. Incubation: Seal the plate with a sealing film and incubate at 37°C for 30 minutes.

4. Prepare the solution: Dilute the 30x concentrated wash solution 30x with distilled water and set aside.

5. Wash: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with wash solution, let it sit for 30 seconds, then discard. Repeat this process 5 times and pat dry.

6. Add enzyme: Add 50 μl of enzyme-labeled reagent to each well, excluding the blank well.

7. Incubation: Perform the same procedure as in step 3.

8. Wash: Perform the same procedure as in step 5.

9. Color Development: Add 50 μl of Color Developing Reagent A to each well, followed by 50 μl of Color Developing Reagent B. Gently shake to mix thoroughly. Incubate at 37°C in the dark for 10 minutes.

10. Stop: Add 50 μl of Stop Solution to each well to terminate the reaction (the blue color will immediately turn yellow).

11. Measurement: Use the blank well as a zero value and measure the absorbance (OD) of each well at 450 nm. Measurements should be performed within 15 minutes after adding the Stop Solution.

Calculation


Draw a standard curve on graph paper with the concentration of the standard as the horizontal axis and the OD value as the vertical axis. Find the corresponding concentration of the sample from the standard curve based on the OD value of the sample; then multiply it by the dilution factor. Alternatively, use the concentration of the standard and the OD value to calculate the linear regression equation of the standard curve, substitute the OD value of the sample into the equation to calculate the sample concentration, and then multiply it by the dilution factor to obtain the actual concentration of the sample.

Standard Curve

Usage

Specimen Requirements


1. Specimens should be extracted as soon as possible after collection, following the relevant literature. Experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, specimens can be stored at -20°C, but repeated freeze-thaw cycles should be avoided.

2. Samples containing sodium nitrate (NaN3) cannot be assayed, as NaN3 inhibits horseradish peroxidase (HRP) activity.

Procedure


1. Dilution of Standard: This kit provides one standard sample at full strength. Users can dilute the sample in a small test tube according to the following chart. 10μg/L

Standard No. 5

Add 150μl of the original standard to 150μl of the standard diluent

5μg/L

Standard No. 4

Add 150μl of the original standard to 150μl of the standard diluent

Standard No. 3

Add 150μl of Standard No. 4 to 150μl of Standard Diluent

1.25μg/L

Standard No. 2

Add 150μl of Standard No. 3 to 150μl of Standard Diluent
Species Reactivity Mouse
Theory This kit uses a double-antibody sandwich assay to measure mouse type III collagen propeptide (Pro-C3) levels in samples. A microplate is coated with purified mouse type III collagen propeptide (Pro-C3) antibody to create a solid-phase antibody. Type III collagen propeptide (Pro-C3) is then added sequentially to the antibody-coated microwells. This antibody then binds to HRP-labeled type III collagen propeptide (Pro-C3) antibody, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the assay is developed with the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of type III collagen propeptide (Pro-C3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader, and the concentration of mouse type III collagen propeptide (Pro-C3) in the sample is calculated using a standard curve.
Detection Type Used to determine the content of type III collagen propeptide (Pro-C3) in mouse serum, plasma and related liquid samples.
Composition

Serial number

Component name

Specification

Serial number

Component name

Specification

1

30 times concentrated detergent

20ml×1 bottle

6

Developer B solution

6ml×1/bottle

2

Enzyme Labeled Reagent

6ml×1 bottle

7

Stop Solution

6ml×1 bottle

3

Enzyme-coated plate

12 wells ×8 strips

8

Standard (20 μg/L)

0.5 ml ×1 bottle

4

Sample diluent

6 ml ×1 bottle

9

Standard diluent

1.5ml×1 bottle

5

Developer A Liquid

6ml×1 bottle

10

Sealing film

2 photos
General Notes 1. After removing the kit from the refrigerator, allow it to equilibrate at room temperature for 1 hour before use. If the enzyme-coated plate is opened and not completely used, store it in a sealed bag. 2. Crystals may form in the concentrated wash buffer. Warming in a water bath can aid dissolution during dilution. This will not affect the results. 3. Use a pipette for each sample addition step and frequently calibrate its accuracy to avoid experimental error. The time for each addition should ideally be within 5 minutes. For large numbers of samples, using a dispenser is recommended. 4. Develop a standard curve with each measurement, preferably with replicates. If the analyte content in the sample is too high (the sample OD value is greater than the OD value of the first standard well), dilute the sample a certain number of times (n times) with sample diluent before measurement. When calculating the final dilution, multiply by the total dilution factor (×n×5).

5. The sealing film is for single use only to avoid cross contamination.

6. Please store the substrate in a dark place.

7. Strictly follow the instructions for operation. The test results must be determined based on the readings of the microplate reader.

8. All samples, washing solutions and various wastes should be treated as infectious materials.

9. Components of different batches of this reagent must not be mixed.
Storage Temp. Unopened test kit, stored at 2-8°C, has a shelf life of 6 months.
Test Range 0.3μg/L-12μg/L
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SKU: 16284814085

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pandajama
Belleville, US
★★★★★ 5
This is a good very distant overview
Format: Paperback
Sometimes I need a really simplistic overview of an entire subject before I feel comfortable sitting down to learn it. Like if you are going to learn about, say, 13th Century France you might want to know just a little about the major issues of the entire century before sitting down with a text book. This book is good for that. It's short, it gets you started with the main keywords, and at the end you have a good picture of what the language can do. And you can get to the end in a day or two. It's very well written, well organized, clear, and very very limited in scope. But if you read it, you will be in good shape to start getting down to business with working with SQL with a more advanced text. This book, in my opinion, was well worth the money and the read even though ultimately I turned to something far more advanced.
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Reviewed in the United States on October 12, 2024
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Matthew Holtsby
Birmingham, US
★★★★★ 5
Great For Beginners
Format: Paperback
Easy to follow, concepts clearly explained... Great for beginners!
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Reviewed in the United States on December 22, 2025
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idris
Whiting, US
★★★★★ 5
Awesome book
Format: Paperback
Its a good book, trust me you wont regret it.
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Reviewed in the United States on April 28, 2026
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Big Fat Mike
Massapequa, US
★★★★★ 3
Does a good job, but too much philosophy.
Format: Kindle
I'm about halfway through this book. I have a condition that makes it hard to read. This book does a good job of teaching SQLite but spends too much time stressing the importance of comments and style. I agree, that these things are important, however, not to the audience of the book. The reader would have been better served by getting into the grit of SQL syntax and which keywords to use when versus ensuring that every query had a comment block with name, date, and description. Moreover, requiring every reader to download a prewritten database to work from and special utilities to follow along takes away from the learning experience. That said, you will learn the basics about SQLite, and by the end of the book be able to handle most problems you'll encounter when developing an application.
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Reviewed in the United States on November 9, 2020
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TigerFL
Lake Worth, US
★★★★★ 5
Great book!
The free promotional items the company gives you just for buying this product alone make it worth the five stars I have given it, and the product itself is amazing. I should start by noting that I received this product about 5 days ago and I am currently halfway done with it. This is not a book that teaches you the very basics of computer programming and assumes that you already have a basic grasp of the fundamentals such as arrays which you definitely should if you are even the slightest bit interested in learning SQL. The book begins by teaching you about what the program is, how it is becoming a valued skill in the world, and why it is a better programming language than others. It makes a language that most people consider highly advanced and more and more difficult the more you use it very easy to grasp in a way that could potentially change your entire career. I am not a computer science expert myself and have only a basic understanding of programming language, mostly java, but I was able to read this book and understand almost all of it without a problem. My computer science friends are even surprised by how much I was able to learn about this programming language that they consider impossible in such a short amount of time. I highly recommend this product if you have any interest at all in learning a new programming language or just giving yourself another skill because they make it very simple to learn. Disclosure: I received this product for free or on a promotional discount for my honest review and they are 100% my own opinions. However everyone has their own opinion on this product. I did not receive payment other than receiving the product at a discounted rate and I am not required to give a good review. I know how important my reviews are to people who are interested in buying this product, because everyone should be reading reviews before they purchase a product. What I include is what I actually think about the product and my opinion alone. My reviews accurately reflect my views on this product and any 5 star product I would recommend to both my friends and family as well as purchase at full price. This disclosure is in accordance with the Federal Trade Commission’s Guidelines on Testimony and Advertising. I provide honest reviews that describe the product it entirety both the positive and negatives (if there are any). If my review was helpful to you, would you please vote yes!
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Reviewed in the United States on May 13, 2016

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