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Description
LR3-IGF-I Protein, SalmonProduct Specification Species Salmon Synonyms LR3 Insulin like Growth Factor I, MGF, IGFI, IGF1A, IGF, IBP1 Accession Q91476 Amino Acid Sequence Gly19 Ala88 (Glu21Arg) Expression System E. coli Molecular Weight 10 12 kDa (Reducing) Purity >95% by SDS PAGE & HPLC. Endotoxin <0. 1EU g Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized powder Storage Buffer PBS, pH7. 4 Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in
Product Specification
| Species | Salmon |
| Synonyms | LR3 Insulin-like Growth Factor-I, MGF, IGFI, IGF1A, IGF, IBP1 |
| Accession | Q91476 |
| Amino Acid Sequence | Gly19-Ala88 (Glu21Arg) |
| Expression System | E.coli |
| Molecular Weight | 10-12 kDa (Reducing) |
| Purity | >95% by SDS-PAGE & HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Endocrinology. 1999 Oct;140(10):4699-705. |
Background
IGF-I is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-I is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-II, which is also a major fetal growth factor. Salmon Long R3 Insulin-like Growth Factor-I contains a 70 amino acid analog of Salmon IGF-I. Compared to the complete IGF-I sequence, an addition of the LR3-IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3) and a 13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of Long R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.
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