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Description
PSCA His Tag Protein, MouseProduct Specification Species Mouse Synonyms Prostate stem cell antigenUNQ206 PRO232PRO232 Accession P57096 Amino Acid Sequence Leu21 Asn95, with C terminal His Expression System HEK293 Molecular Weight 20 30kDa (Reducing) Purity 95% by SDS PAGE &90% by SEC HPLC Endotoxin <0. 1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer PBS, 5% trehalose, PH7. 4 Reconstitution Reconstitute at 0. 1 1 mg ml according
Product Specification
| Species | Mouse |
| Synonyms | Prostate stem cell antigen，UNQ206/PRO232，PRO232 |
| Accession | P57096 |
| Amino Acid Sequence | Leu21-Asn95, with C-terminal His |
| Expression System | HEK293 |
| Molecular Weight | 20-30kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE &＞90% by SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, 5% trehalose, PH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Reiter RE, Gu Z, Watabe T, Thomas G, Szigeti K, Davis E, Wahl M, Nisitani S, Yamashiro J, Le Beau MM, Loda M, Witte ON. Prostate stem cell antigen: a cell surface marker overexpressed in prostate cancer. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1735-40. |
Background
Prostate stem cell antigen (PSCA), a cell-membrane glycoprotein composed of 123 amino acids, is frequently expressed in prostate cancer. Mouse PSCA (mPSCA) exhibits 65% homology with human PSCA at both the nucleotide and amino acid levels. The knockdown of PSCA has been shown to induce epithelial-mesenchymal transition (EMT) and to reduce the metastatic potential of DU145 cells, indicating a significant role for PSCA in the carcinogenesis and progression of prostate cancer.
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