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Description
Mouse PECAM1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Platelet/Endothelial Cell Adhesion Molecule (PECAM1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Platelet/Endothelial Cell Adhesion Molecule (PECAM1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Platelet/Endothelial Cell Adhesion Molecule ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Platelet endothelial cell adhesion molecule 1 (PECAM1) is a protein normally found on endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, lymphocytes (T cells, B cells, and NK cells), megakaryocytes, and osteoclasts. The encoded protein is a member of the immunoglobulin superfamily and may be involved in leukocyte migration, angiogenesis, and integrin activation. PECAM1 on endothelial cells binds to the CD38 receptor on natural killer cells, allowing these cells to attach to the endothelium. It plays a key role in clearing senescent neutrophils from the body. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.7 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 1
Disgusted!
Size: Small, Color: Black
I’m disgusted! I ordered this for my boyfriend to wear to prom. I ordered him a small and the jacket and the vest were not smalls, they look like they were a large. All three pieces had dog or cat hair on them and the jacket had dirt stains on the inside. They were all used! And some of the stitching was coming out! I’m so glad I received it the same day I bought it, because if received it closer to prom I would have been so screwed!!! Do not buy this or buy from this brand! Terrible product!!
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Reviewed in the United States on April 10, 2026
★★★★★ 5
Dress to Impress: Sharp, Tailored Fit, Quality Material, and Sizing Tips
Size: X-Small, Color: Navy Blue, Size: X-Small, Color: Navy Blue
This review is for the LUPURTY Men's Suits Slim Fit, 3 Piece Suits for Men, 2 Buttons Solid Jacket Vest Pants, Tuxedo Set (XS navy blue and black sets).
I bought the XS knowing my son's measurements were actually below the size guide for that size, but I'm glad I did because it still worked out. The suit was a bit big—especially in the pants, which were quite long, and slightly roomy in the shoulders and arms—but it still looked sharp and well put-together. The overall fit has a clean, tailored appearance despite the extra room. The material feels high-quality and the color is true to what’s shown online. I do wish the black suit came with a black or gray tie instead of blue, which didn’t quite match.
The suit in the photos is the navy blue set that my son wore to his 8th grade graduation, which matched their school colors perfectly.
I also included my son’s measurements to help others with sizing:
Weight 114 lbs
Height 5'3"
Chest 30.5"
Waist 29"
Hip 35"
Sleeve length 21.5"
Shoulder 17"
Inseam 29"
Back length 18.5"
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Reviewed in the United States on July 9, 2025
★★★★★ 5
Class without the price
Size: Large, Color: Burgundy
After purchasing many of the suit offerings on Amazon, Luperty's suit are the best for the price. The adjustable pants makes for a smooth fitting experience, and allow you to breathe when you've clearly eaten to much. The pant length is ideal for a classic suit appearance. If you want a more modern fit you'll have to get them hemmed higher. The vest is form fitting, which is to say not as forgiving as the pants, but will definitely cut a sharper figure. The jacket fits well off the rack for me, albeit the sleeves could be pulled in a bit. I ordered a Large size, which all the pieces fit well for a 5'11", sub 200 lb man. I wouldn't say I'm very in shape, so your milage may very.
After buying my first suit from Luperty, I was so impressed that I purchased the rest of the colors as well. My only complaint is that I wish the sleeve lining was more interesting. They all use the same generic white striped fabric. I would have liked to see more variation for each of the different colors.
As I have bought all the colors, even the cream color which they no longer offer, I think I can give some insight on which ones work best.
1. Burgundy - This is the best looking one. It's a statement to wear this color of suit. This suit is bold but refined, like a smooth wine. I've gotten the most compliments with this color than any other. It's eye catching and sharp, and you'll feel classy in it. I've actually bought two of these.
2. Dark Grey - This suit is the one you wear to an important board meeting. It's a beautiful charcoal that has a nice sheen to it. A timeless appearance that shows class but muted enough to not take center stage.
3. Cream - Another bold suit, maybe too bold seeing as how they no longer sell it. This suit defines the Miami Vice mafioso look, and it pulls it off nicely. Pairs extremely well with bright pastels. Don't forget to lose the tie and unbutton at least two of the top buttons to get the full effect.
4. Navy Blue - This one's name does it a disservice. The blue is almost at Royal Blue level. The color is definitely striking and can be elevated easily. You might be able to pull this off in a professional setting as well as blue is seen as a more business oriented color, though I believe this is just slightly over the top. However, I like over the top.
5. Light Grey - This one is very light. You wear this one to the same things as the dark grey one, but want to show a bit more character. It strikes me as more of a European color, and maybe that makes it feel more sophisticated. Wearing it with a pocket watch is optional but recommended.
6. Black - This is where we start to decline in color quality. I knows it's black, but I'd like to have seen some more texture, similar to the dark grey. Hopefully you don't have to wear it much given the negative context.
7. Dark Navy - I think I like this one better than the black one, however this one is DARK. I often have to put it right next to my black one to differentiate them, it's that dark. In various lighting you can tell people its a black suit and no one would refute you. Because of this reason I think it's worse than the black. But it makes for the perfect bog standard suit to wear into the office if you need it to.
All of these suits are under $100, and often go on sale for less. I cannot recommend them enough for first time suit buyers.
As always, remember to open up the vents in the back that are sewn shut, as well as the pockets. As lucky as I am to be able to have these fit almost perfectly, the last step in any suit journey is to have them tailored to you. It might cost as much as this suit, but a $1000 suit off the rack will have nothing on a $100 suit that's been tailored.
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Reviewed in the United States on June 2, 2025
★★★★★ 5
Premium Quality at an Affordable Price
Size: Large, Color: Dark Navy, Size: Large, Color: Dark Navy
I recently purchased this men’s suit and was genuinely impressed with the quality and fit. The fabric feels premium, comfortable, and well-stitched, giving it a classy and elegant look. The fitting was true to size. The color and design matched the pictures exactly. Highly recommended for anyone looking for a stylish and affordable suit.
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Reviewed in the United States on May 9, 2026
★★★★★ 5
Great quality for the price.
Size: Large, Color: Black
All I have to say about this suit is WOW! I lost a good chunk of weight and needed a new suit for an event. Given the price I gave this a shot, and I am really impressed with the quality for the cost. The size is perfect, and the pants are comfortable with an internal elastic that looks and feels nice. The design makes it so the pants don’t slip down with nylon and silk shirts. Looks just as good (if not better) than suits in the $300 price range. I can’t speak for the other colors, but the black suit is exactly what I needed. Let’s see how long the suit lasts!
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Reviewed in the United States on April 11, 2026
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