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Description
Rat CASR ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 500pg/mL). Then dilute to the following concentrations: 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, 7.8125pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 500pg/mL standard working solution into the first EP tube and mix thoroughly to make a 250pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a calcium sensing receptor (CASR) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the calcium sensing receptor (CASR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Calcium Sensing Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The calcium-sensing receptor (CaSR) is a class C G protein-coupled receptor that senses extracellular calcium levels. It is primarily expressed in the parathyroid glands, renal tubules, and the brain. In the parathyroid glands, it controls calcium homeostasis by regulating the release of parathyroid hormone (PTH). In the presence of elevated plasma calcium concentrations and calcium receptor activation, PTH release is inhibited. Increased extracellular calcium binding induces a conformational change in the receptor, which activates the phospholipase C pathway intracellularly, presumably through Gqα-type G proteins. This ultimately increases intracellular calcium concentration and inhibits vesicle fusion and extravasation of parathyroid hormone. It also inhibits cAMP-dependent pathways. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 7.81-500 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 6 reviews
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Product Reviews
★★★★★ 5
Excellent adjustable drafting chair
Size: 934-Z, Color: Blue
Excellent chair design, extremely comfortable and exceptionally easy instructions to follow making for simple assembly. Love the amply wide cushioned seat and ergonomic backrest. The height adjustment feature works perfectly as well as positioning of the foot ring . Having the ability to raise the arms is also a big plus! I highly recommend this product for attractive appearance, comfort features, functionality and operation. Really nice chair!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 13, 2026
★★★★★ 4
Comfortable, stylish, and perfect for long work hours 💗✨
Size: 934-Z, Color: Pink
I bought this chair because I spend a lot of time working and needed something comfortable but also stylish, and I absolutely love it.
Since I started using it, I feel much better support in my back thanks to the lumbar support. It makes a big difference, especially during long work hours. The adjustable footrest is also a feature I didn’t know I needed, but now I use it all the time.
The flip-up armrests are very convenient because I can adjust them depending on what I’m doing or move them out of the way when needed.
The pink color is beautiful and adds a soft, elegant touch to my workspace. It looks great in a modern home office.
It feels sturdy, well-made, and very stable. Overall, it’s a great purchase that improved both my comfort and the look of my workspace.
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Reviewed in the United States on April 29, 2026
★★★★★ 5
All-around thumbs up!
Size: 934W-Z, Color: Black
So far, so awesome. Easy to assemble, comfortable, arrived quickly and is exactly what I was looking for. I have a tall work bench in my new studio so I needed a chair that we could allow me to sit higher than a regular office chair and allow me to get off my feet after standing on the concrete floor. Plus its a nice looking chair that I am sure will last a long time. I love the flip-up arms and the lumbar support. This is just right!
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Reviewed in the United States on May 8, 2026
★★★★★ 5
Perfect Balance of Comfort, Support, and Style!
Size: 934-Z, Color: Black, Size: 934-Z, Color: Black
I’ve been using this chair for a few weeks now, and I’m genuinely impressed with how much of a difference it’s made in my workspace. The mesh back is one of my favorite features—it offers excellent breathability and prevents that sticky, overheated feeling you sometimes get with solid-back chairs, even after sitting for hours. The seat cushion is firm enough to give proper support but still has enough give to keep me comfortable through long workdays. It feels like the perfect middle ground between soft and supportive.
The adjustable foot ring has been a complete game changer for me. I use a taller desk setup, and having somewhere to rest my feet makes a huge difference in posture and overall comfort. Whether I’m sitting upright for focused work or leaning back to read, it’s always at the right height.
The flip-up armrests are another smart design choice. When I’m typing, I like to have them down for support, but when I’m done, I can flip them up and push the chair neatly under my desk—saving space and keeping things tidy. The casters roll smoothly on both hardwood and rugs, so I can move around easily without scraping the floor or getting stuck. The overall build quality feels solid and sturdy, with no wobbles or creaks.
Assembly was surprisingly quick—everything was clearly labeled, the parts felt durable, and it only took me about 20 minutes from opening the box to sitting in the chair. The sleek, modern look is another big plus—it blends in perfectly with my home office décor without feeling bulky or taking up too much visual space.
If you’re looking for a chair that combines comfort, ergonomic support, durability, and style, I can’t recommend this one enough. It’s equally great for work, studying, or just relaxing at your desk, and it’s definitely been worth every penny.
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Reviewed in the United States on August 12, 2025
★★★★★ 3
OK for the price, but overall comfort is avergae
Size: 934-Z, Color: Mustard
Easy to put together, color (mustard) accurate to images, and while I understand this is not a high-end chair, I feel like it still needs to be rated for its quality.
Pros: the seat cushions are fairly comfortable and it rolls well. Adjustable footrest is easy enough and mostly sturdy.
Cons: For a tall office chair, it doesn't go up high enough. I actually had to lower my standing desk to sit at it, which then isn't where I want it when I'm standing now. It's also a bit wobbly. The seat area's attachment to the post isn't as solid as it should be for a tall chair, so while sitting, it does tend to lean/slope forward and wobble side to side. The lumbar support is not adjustable, so I'm not even really sure what it does. And the armrests are not very comfortable to use. Since they are hard plastic, they are just a bit too rigid to rely on for any comfort. They are basically there to hold on to when you're getting out of the chair.
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Reviewed in the United States on May 29, 2026
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