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Description
Human MMP17 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a matrix metalloproteinase 17 (MMP17) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of matrix metalloproteinase 17 (MMP17) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Matrix Metalloproteinase 17 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Matrix metalloproteinase 17 (MMP17), also known as membrane-type matrix metalloproteinase 4 (MT-MMP 4), is an enzyme encoded by the MMP17 gene. Proteins in the matrix metalloproteinase (MMP) family are involved in the breakdown of the extracellular matrix during normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins and become activated when cleaved by extracellular proteases. The protein encoded by this gene is considered a member of the membrane-type MMP (MT-MMP) subfamily. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
The Perfect Sheets To Lie On While Writing Amazon Reviews
Size: Full, Color: Light Gray
As I write this, I am laying on an adjustable full sized bed by Hofish and a 10.5" comfort foam mattress by Classic Brands. I know I sound like I am an Amazon plant, but I swear I am not. I'm not even a stockholder anymore.
This is the most awesome bed stylings combination I have had in many, many years. In fact, it is the best I have ever owned and it is beat only by some ritzy resort I stayed at on vacation like 20 years ago, though I admit I was pretty drunk the entire time so my memory might not be the most accurate.
But getting back to today... these sheets are great. I washed them twice - I'm not a neat freak, I am just amazingly disorganized and easily distracted while doing laundry, so tonight they went though two wash cycles and one dry. And then I put them on the bed. While these sheets are not deep pocket sheets, they don't need to be with this mattress. There is enough room and border for them to tuck and wrap with the elastic to give a strong grip under the mattress, which is important as the adjustable frame continually put the sheets and mattress though its repeated adjustable calesthenics-type positioning. The other two sheet sets I have used since I got this bed have struggled poorly with this. In the day or so and at least a dozen position changes, these sheets stay in place with no popped corners to exposing the bowl as the head and feet rise. Of course, till will tell if it lasts, but so far they are the best of the batch of sheets in my closet.
As for texture, they are awesome. I'm a dude. I don't really know what thread count or knit or whatever really means. But these sheets feel like a well-washed favorite, quality rock & roll concert t-shirt. And not one of these glitter puff stupid new groups, like a real rock band from the 80s that now charges $250 for a concert ticket and $60 for a t-shirt...but that group you saw back then, paid $10 for the shirt and you STILL have it and love to wear it because it is perfectly molded to your body, soft and reliable. The kind of shirt you will wear under your suit at work and just grin not just for mental middle finger to corporate dress codes, but because the t-shirt is just that great of a feeling.
Well, that is what these sheets feel like. And now you get to sleep in it. It is that awesome.
In LA right now we are having a nasty heat wave, but these sheets don't seem to bake me even with my stuttering AC. The flannel sheets I love seem to trigger the butt sweats as soon as I lay down in this heat, but no such problem with these.
So really, I'm absolutely happy with these things. I am going to order more in other colors as soon as Amazon gets them back in stock. Great price, great quality, great comfort, great fit, great deal. It is that simple.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 3, 2017
★★★★★ 5
Soft smooth and wrinkle free!
Size: King, Color: White
The most comfortable sheets I’ve had in years! Soft and don’t wrinkle up in the bed. Some reviews said they are thin but I think they’re perfect! I don’t want super thick sheets as we are hot sleepers. These are the perfect feel. They also stretch very well to fit on my mattress all the way around and I have a thick mattress! My precious sheets were a struggle to get over the corners but these fit perfectly. Would buy 10 times over! Such a good value as well!
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Reviewed in the United States on April 14, 2026
★★★★★ 4
Soft Material, but Color Not as Expected
Size: Queen, Color: Oatmeal, Size: Queen, Color: Oatmeal
I did not open or use this sheet set because the color wasn’t suitable for the occasion I intended. I prefer to return items in unused condition so they can be resold as new, so I cannot comment on the exact fit. From a price point, it's value for money.
From touching the fabric through the packaging, the material feels soft and of fair quality. There is no unpleasant smell, and the overall functionality seems fine based on its construction.
My only disappointment is the oatmeal color—it looks somewhat dull and gives off an aged or slightly used appearance. I was hoping for a brighter, creamier tone. Aside from the color issue, the product appears to be well made and comfortable, which is why I’m giving it four stars.
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Reviewed in the United States on December 6, 2025
★★★★★ 5
Nice and soft
Size: Queen, Color: Chambray
Update: I’m very happy with this sheet set! I’m currently only using the fitted sheet as I don’t have a need for the top sheet or pillow cases right now. It hasn’t popped off the mattress at all like the previous cheep sheet I had.
Original post: The sheet set arrived today. Haven’t actually tried it yet because I need to wash it first. From I can see, looks great. Once it’s washed and on my bed, and can figure out how to update my review I will do so accordingly. It’s soft like most people have said and the color is nice, though they didn’t have my favorite color purple in a shade I like so I got the ‘fancy blue’ (don’t remember the actual name of the color) because I desperately needed a new sheet of quality.
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Reviewed in the United States on March 1, 2026
★★★★★ 5
Very comfy sheets, nice fabric
Size: Full, Color: Oatmeal
I wasn’t expecting such good quality forthis price. They’re very soft, but of fairly thick fabric. I’m sleeping great on them. The pockets are deep enough for an 8” foam mattress plus my 3” topper. Will be getting another set for the second bedroom.
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Reviewed in the United States on April 13, 2026