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Description
Organotal rat normal colon organoid culture KitProduct Specification Usage 1. Primary (1) the normal colonic tissue of rats after sampling must be cleaned at 2 8 , several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the Petri dish, wash it with primary culture buffer B for three times, remove impurities, remove the intestinal mucosa of normal colon tissue of rats
Product Specification
| Usage |
1. Primary (1) the normal colonic tissue of rats after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the Petri dish, wash it with primary culture buffer B for three times, remove impurities, remove the intestinal mucosa of normal colon tissue of rats with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm3 (3) the tissue was digested with rat normal colon primary tissue digestive solution C, and then shaken at 4 ℃ for 20-25min or left to digest for 40-50min (observe the digestion at any time during the digestion process) (4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion (5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form glue, and add rat normal colon organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of rat normal colon organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of rat normal colon organoid culture medium A. |
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| Synonym | Rat Colon Organoid Culture Kit | ||||||||||||||||
| Description | Composition:
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| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
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4.3 ★★★★★
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Product Reviews
★★★★★ 5
It's not glamorous but it works wonders - even while I am on Accutane!
Size: 19 Ounce (Pack of 1)
This is the best, most luxurious lotion I have ever used. And the only lotion that I can even stand to be on my skin.
I started Accutane (for acne) last year and was told that my skin would dry out horribly and that I needed to find a good moisturizer to limit the peeling and cracking that I would inevitably experience. Prior to the medicine my skin was oily and very acne prone and every lotion that I had tried either made me feel like I was wearing a latex suit or broke me out horribly. After doing some research I read that this was a great solution and figured I would give it a try. I started it out right away to try to avoid getting dry and was shocked at how well it worked. Even at the beginning when I was still oily this lotion never felt too heavy. Now don't get me wrong-it goes on like frosting and initially feels like a lot-but within minutes it soaks in and leaves your skin feeling like a little kids (not the sticky kind of little kid though-the good clean little kids with undamaged skin). It will last for days too. No need to reapply because it has soaked in to your skin and doesn't just sit on the surface. My skin has never been this soft. And here I am, nearly 9 months in to my Accutane medication regimen and I am not flaking or peeling. In fact, my skin has never looked better or more healthy. My doctors are shocked at how my skin has stayed moisturized because nearly everyone peels. And it doesn't break you out which is obviously something super important. Now, could I be an anomoly? Maybe, but I don't think so. I honestly think this lotion is Just. That. Good! The price isn't bad either!
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Reviewed in the United States on February 5, 2026
★★★★★ 5
Great for dry skin and the whole family
Size: 19 Ounce (Pack of 1)
We use this at home for everyone, including the kids, and it works really well. We all have pretty dry skin, and this cream keeps it hydrated for hours. I apply it after showering and it leaves the skin soft without feeling greasy. It has no strong scent, doesn’t irritate, and hasn’t caused any breakouts. I also feel comfortable using it on my kids because it’s gentle and effective. The jar lasts a long time since a little goes a long way. I would definitely buy it again.
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Reviewed in the United States on March 28, 2026
★★★★★ 5
Dry Skin Miracle!
Size: 8 Ounce
I have very dry skin, and around my chin and nose, it flakes and turns red, exposing my inner layer of skin due to severe dryness. I could never seem to hydrate it or get rid of the flakiness. I thought it was just my skin at this point, and I could do nothing to solve it. Now, I've never considered using Cerave; I've always heard negative things about it. However, I've never been a makeup person until now, when I want to try to elevate my look (not to mention I'm always in pain from a lack of hydration). The problem is, my skin is so dry that it cracks the makeup, preventing it from setting well on my face.
Another problem is that around my nostrils, the flakiness looks like boogers, nasty. It's incredibly annoying. So I did a little research, and now I'm here. LET ME TELL YOU! This cream is amazing! I love how thick it is, and how a little goes a long way! My face, for the first time in my short life, is not dry! It's not buttery smooth, as in your hand won't glide against my face, but it won't feel the flakiness and roughness of my skin—and that's a game changer! I'm genuinely obsessed with randomly rubbing my face to feel the almost barrier of hydration, especially around my nostrils! I cannot explain the level of amazement I felt when I touched my nostril area to feel practically smooth skin. I felt as if it were a miracle. I've only used it for a week now, keep in mind, and this is how I feel. PLEASE BUY THIS IF YOU'RE LIKE ME! I'M SO HAPPY WITH THIS PURCHASE, AND IT'S WORTH ALL MY MONEY 🙏
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Reviewed in the United States on March 6, 2026
★★★★★ 5
From dry to soft in days.
Style: 3.38 Fl Oz (Non SPF)
I bought this moisturizer at a time when my skin truly needed help, with mature and sensitive skin, and I was dealing with intense dryness. My skin felt tight, dull, and uncomfortable, even after trying several products that didn’t provide lasting hydration.
I decided to try this cream and within the first few days, I noticed a real difference. It’s lightweight, absorbs quickly, doesn’t feel greasy, and most importantly, it doesn’t irritate my skin—instead, it soothes it. With continued use, my skin became softer, more supple, and visibly healthier, without that constant tight feeling.
I love that it’s fragrance-free, oil-free, and non-comedogenic, as it hasn’t caused any breakouts. It also contains ceramides and niacinamide, and the added SPF makes my routine much easier. After trying many products, I can honestly say this is the only one that truly worked for me. It’s now a daily essential.
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Reviewed in the United States on April 18, 2026
★★★★★ 5
Great daily lotion
Style: 3.38 Fl Oz (Non SPF)
Great daily lotion. I love that it is light and fragrance free. It is perfect for day use. I have dry skin and sometimes it is not enough to use by itself. I mostly use it during the day as a base for sunscreen but it feels really good on the face and does not leave my face feeling greasy. The ingredients are really good and the value for money is great. I love LRP products and this is so easy to use.
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Reviewed in the United States on April 17, 2026
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