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Description
Mouse C3 OneStep ELISA KitProduct Specification Antigen C3 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Ms Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 4. 0% Inter assay: 7. 2% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 8. 854 pg mL Range 31. 25 pg mL 2000 pg mL Recovery Cell culture supernatant: 86%
Product Specification
| Antigen | C3 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.0%； Inter-assay: 7.2% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 8.854 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Recovery | Cell culture supernatant: 86% Serum: 90% Plasma: 116% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse C3 is a central and essential component of the complement system, acting as the pivotal convergence point for all three complement activation pathways: classical, lectin, and alternative. This ~186 kDa protein, primarily synthesized in the liver, is proteolytically cleaved into two key fragments upon activation: the anaphylatoxin C3a, which recruits and activates immune cells, and the opsonin C3b, which tags pathogens for phagocytosis and amplifies the complement cascade. Its critical roles include mediating innate immune defense against infections, clearing immune complexes and apoptotic cells, and bridging innate and adaptive immunity. Consequently, Mouse C3 is a major focus of research in infectious disease, autoimmunity (e.g., lupus), neuroinflammation, and ischemia-reperfusion injury, with genetically modified C3-deficient mice being a fundamental tool for elucidating its precise functions in vivo.
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