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Description
Human ADAM9 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against A disintegrin and metalloprotease 9 (ADAM9). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the amount of A disintegrin and metalloprotease 9 (ADAM9) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human A Disintegrin And Metalloprotease 9 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | A disintegrin and metalloproteinase 9 (ADAM9) is an enzyme encoded by the ADAM9 gene. It is a member of the ADAM (disintegrin and metalloproteinase domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrin and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.1 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 5
Perfect introduction
Format: Library Binding
She loves identifying and finding the dog
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 31, 2022
★★★★★ 5
Made in America nano-hydroxyapatite Toothpaste that's Well Worth Buying.
I've been using the Japanese brand Apagard Premio toothpaste for a couple of years now and now I've tried this Boka toothpaste while looking for alternatives that wold be less costly.
Boka Toothpaste is very mild and makes brushing your teeth better. It doesn't have that harsh mint flavor which with most toothpastes feels like it's burning your mouth. It comes in multiple flavors and when you're done brushing your teeth, there is no need to rinse your mouth as it is a safe toothpaste to use.
After using it for a few days, I feel that I will keep using the Boka brand from now on.
I just wish that most stores would carry this toothpaste instead pf selling all those major brands which are full of fluoride.
Did a bit of research and found that Boka Toothpaste has the same percentage of nano-hydroxyapatite as Apagard premio which is 10%. So that means that Boka Toothpaste is just as good if not better.
No Fluoride
No Harsh burning Mint Flavor
Safe to shallow
Multiple flavors
10% nano-hydroxyapatite
Keeps your teeth Strong
And one thing I've noticed is nano-hydroxyapatite makes your teeth less sensitive by rebuilding your tooth enamel
I ordered four flavors, Watermelon Mint, Strawberry Mango, Orange Cream and Coco Ginger.
So far, I've tried the Coco Ginger and I was surprised at how mild it was and it didn't have the sharp taste that ginger usually has. It is good and I enjoy it. I'm looking forward to trying the other flavors. The Orange Cream I'm sure my wife will like if I can get her to try it. But she can be very stubborn at times.
I see there are some other flavors which I intend to try such as the Cinnamint, Cucumber Dill, Lemon Lavender and Strawberry Basil. Not sure if I should try the Ela Mint as I don't know how strong or mild the mint will be.
I'm sure that if places such as Walmart brick & mortar stores sold this toothpaste, that it would sell really well.
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Reviewed in the United States on May 31, 2025
★★★★★ 5
Best paste and taste
Every once in a while you run into a product of true quality. Time is expensive currency so we’ll keep it concise. This is the best tooth paste we’ve ever tried. All good quality ingredients fluoride free. First thing we noticed is how quick it works, it cleans your teeth super quick used with electric toothbrush cutting brushing time considerably. Leaves mouth feeling clean and smooth plus great taste. A lot of new advanced products in self care tend to have chemical type scents and textures but this tooth paste has a pleasant feel and taste, we got coco ginger. Will buy again.
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Reviewed in the United States on February 16, 2026
★★★★★ 5
Tastes great, works great
I love this toothpaste! I’m so happy to have a toothpaste that isn’t mint-flavored.
FLAVOR: The taste and smell are so good — the coco ginger flavor is delicious and I legitimately look forward to the taste of it. I was worried that the ginger might be strong/spicy, but it’s nicely balanced. The lavender lemon flavor is also pretty good, like a lemon pastry. The elamint flavor tastes like regular mint toothpaste, just maybe slightly more herbal.
SENSITIVITY: I’ve had to use sensitive toothpaste for many years. Any time I switched off it, I would start experiencing sensitivity again within a week or so. I’m really happy to report that this toothpaste offers great sensitivity and enamel protection.
WHITENING: I don’t feel that this toothpaste is contributing much in terms of whitening. My previous toothpaste had gentle whitening, and I do feel like I could tell it was working. While my teeth haven’t gotten less white since I switched to Boka, I don’t think they’ve gotten any whiter either. That’s okay with me — the flavor and sensitivity protection are worth it, and I can always do a whitening treatment in the future if I ever feel I need to.
TEXTURE: The texture is just like normal toothpaste and brushing with it feels the same as any other toothpaste. It works great with both my manual toothbrush and my electric toothbrush.
TUBE: The tube is really nice and sturdy. I like the design of the tube a lot. The text and color scheme are fairly minimalist, and I like that the cap has a wide base so the tube can stand upright. It looks nice on my bathroom shelf. My only small complaint is that it gets harder and harder to squeeze the toothpaste out once the tube is less than half full. Since the tube is so sturdy, it can’t be rolled and folded to get the paste out. I think this issue could probably be resolved easily with one of those squeezing tools, though. I’ll report back if I try it.
SIZES: I love that it’s available in both a regular size and a little travel size. I also loved that the travel size allowed me to try out this toothpaste before committing to a whole large one. (Don’t worry, the tube in my photos was full when it arrived! I’ve used half of it.)
Overall I highly recommend this toothpaste! I will absolutely buy it again.
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Reviewed in the United States on August 23, 2025
★★★★★ 4
Taste Great
This is a great tasting all natural toothpaste that actually does what it claims. My teeth feel so clean and fresh after brushing and the whitening is immediately noticeable, something that caught me by surprise. It's super easy to use and functional like any other toothpaste but without all the foaming at mouth. The ingredients are natural and it's fluoride free. I was looking for more of the ginger to come through but sadly it's hardly noticeable. I do like it but will try the other flavors next time.
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Reviewed in the United States on September 18, 2024