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Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)Product Specification References 1. Mingzhu Yang, et al., Chemical induced chromatin remodeling reprograms mouse ESCs to totipotent like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle enhanced radiotherapy synergizes with PD L1 blockade to limit post surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and
Product Specification
| References | 1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. Ed Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. 7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological studies. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Usage | 1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100. 2. Use of secondary antibody: This kit is matched with a universal secondary antibody for pika and rabbits, which is not suitable for mouse tissues. Please verify whether the species of the primary antibody matches before the experiment. Please refer to (# abs50038) for mouse tissue samples. 3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:abs994) |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Synonym | Multicolor kit |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 480, TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 690, TSA monochromatic fluorescent dye 770, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI. |
| General Notes | 1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management. |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC). |
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4.4 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 5
Excellent.
kubrick's first i think. an excellent b/w noir crime film. very influential. sets the standard. must see.
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Reviewed in the United States on June 5, 2026
★★★★★ 5
2 Early Kubrick Films in 1 Thrilling Package
The most important thing for the buyer to know about The Killing Criterion Blu-Ray is that it collects 2 of Stanley Kubrick’s earlier films: The Killing (1956) and Killer’s Kiss (1955). Even though the Killer’s Kiss title is not on the box, it is present as an extra, along with its trailer.
Other than that, you can expect the usual from a Criterion Collection release. For those who don’t know, that means terrific quality and presentation, with loads of extra supplementary material.
As far as the movies themselves go — unlike Fear and Desire, I can actually recommend these two movies as movies, as opposed to morbid curiosities of Kubrick fanatics. They are both pretty intense crime thrillers and just a ton of fun. This is Kubrick before he makes big studio movies with big budgets, but after he’s gained more of the experience and sophistication he would later be known for. The product feels raw, gritty, sometimes intimate, and gives you a look at his NYC roots. Kubrick is still an underdog at this stage in his career. So, would definitely recommend for Kubrick fans, and just crime thriller fans in general
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Reviewed in the United States on December 11, 2022
★★★★★ 5
The Mastery of Stanley Kubrick
We're all children of Kubrick aren't we? Is there anything that you can do that he hasn't done? - Paul Thomas Anderson.
One of the most true statements about a great director from another great director. Stanley Kubrick's The Killing is a harsh piece of filmmaking that is the true definition of a film noir heist movie. The characters are not glamorous and they are not symphatetic. They are merely real and that is all you need in a movie. These characters move through the intricate plot with the masterful direction of Kubrick guiding them through it and speaking the crisp dialogue from pulp novelist Jim Thompson. With that combination The Killing is noir at its finest. A great early masterpiece from Kubrick. This Criterion Collection blu-ray also includes another early Kubrick work, Killer's Kiss, about a boxer who falls in love with a woman who lives next door to him and thus gets caught up in a dangerous game of cat and mouse with her shady boss. A great companion movie for The Killing. But there is one disappointment with this Criterion release. When it was first annouced that they were releasing this film, I had high hopes that they would release the rare Kubrick film Fear and Desire with this, but I was sadly disappointed. Maybe there is a seperate Criterion release for that film in the near future, we can only hope. But all in all this is a great blu-ray, highly recommended for fans of Kubrick or fans of the film noir genre.
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Reviewed in the United States on October 19, 2011
★★★★★ 5
Stanley Kubrick is a talented director.
If you are interested in the director Stanley Kubrick, you will enjoy his process of direction in this film from 1955.
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Reviewed in the United States on January 25, 2026
★★★★★ 5
Best glass skin mask that makes it look like you just had a facial!
Color: Lifting (Jericho Rose), Color: Lifting (Jericho Rose)
I bought this mask yesterday and used it last night for the first time. It instantly became part of my skincare regimen after just one use. I’ve tried a couple of different Korean skincare masks, but this one is already my top pick. First, it comes with the silicone applicator to help apply it evenly. It’s not sticky or tacky and it stays in place. The directions say wait 40 minutes and after 40 minutes it is dry and peels off like a normal face mask! It doesn’t pull at your skin when peeling it off, you don’t need to rinse it with water, and then the results are INSTANT. I was completely captivated in the shine it gave my skin. This will be a repeated purchase which sounds crazy after one use, but you can just tell it’s that good!! I included before and after (less than an hour time difference in these photos!)
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Reviewed in the United States on March 6, 2026