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Description
Alexa Fluor® 647 Mouse Anti-Human CD5 Antibody (S-576-35)Product Specification Host Mouse Antigen CD5 Synonyms T cell surface glycoprotein CD5, Lymphocyte antigen T1 Leu 1, LEU1 Immunogen Recombinant Protein Location Cell membrane Accession P06127 Clone Number S 576 35 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Purification Protein G Concentration 0. 2mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 0. 1% BSA, 0. 01% Proclin 300 Stability & Storage
Product Specification
| Host | Mouse |
| Antigen | CD5 |
| Synonyms | T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, LEU1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P06127 |
| Clone Number | S-576-35 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 0.2mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume |
Background
CD5 is a cluster of differentiation expressed on the surface of T cells (various species) and in a subset of murine B cells known as B-1a. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. There is no confirmed ligand for CD5 but there is evidence that CD72, a C-type lectin, may be a ligand or that CD5 may be homophilic, binding CD5 on the surface of other cells.
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