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Description
Human LPO ELISA KitProduct Specification Usage Experimental equipment required: 1. Microplate reader (450nm) 2. High precision pipette and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled or deionized water Sample processing and requirements: Breast milk tearsOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre test preparation: 1. Remove the test kit from the refrigerator 10
Product Specification
| Usage |
Experimental equipment required: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled or deionized water Sample processing and requirements: Breast milk tearsOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a lactoperoxidase (LPO) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of lactoperoxidase (LPO) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Lactoperoxidase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Lactoperoxidase is a peroxidase secreted from the mammary glands, saliva, and other mucosal glands (including the lungs, bronchi, and nose). It functions as a natural first line of defense against bacteria and viruses. Lactoperoxidase is a member of the heme peroxidase family. Lactoperoxidase is encoded by the LPO gene. Lactoperoxidase catalyzes the oxidation of several inorganic and organic substrates with hydrogen peroxide. These substrates include bromide and iodide, thus classifying lactoperoxidase as a haloperoxidase. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Breast milk, tears, and other biological fluids |
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4.7 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
Finally Peter gets to grow up
Format: Kindle
Amazing art and story that is down right fun to read. Fans finally get to see the adult Spiderman deal with crime, bad guys and of course his family- the wife and kids.
Its also gives a new spin on all of Spiderman’s cast of both friends and enemies.
Don’t pass up on this one.
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Reviewed in the United States on October 8, 2025
★★★★★ 5
Fantastic Fresh Take!
Format: Paperback
Im surprised this book has not been spoiled all over the internet, with how phenomenal and unique it feels to read!
This has been the best in the Ultimate universe in following up the Ultimate Invasion short series that kickstarted this universe!
Hickman's found a way to nuance Peter's dialogue for the older and less experienced age he is coming in with! The fights and exchanges are oddly unlike any of the millions of takes on this character.
The stakes and surrounding family feel new and so exciting to read!
Humongous fan of the morals being touted and explored here!
The book always looks incredible!
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Reviewed in the United States on December 7, 2025
★★★★★ 5
One of the best Books!
Format: Paperback, Format: Paperback
Love all things spider-man! while i’m all caught with this book, i wanted this to be the first comic ever owned! can’t wait to purchase the next volumes (sucks this run and the others are ending)
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Reviewed in the United States on February 12, 2026
★★★★★ 5
Unbelievably Mind Blowing
NO SPOILERS REVIEW!!
I just do not have words to describe this comic novel. I may need too much words to put forth my review about this book.. but then I do not want to spoil anything for the readers. So let me try....
Story
Amazingly written and I am not surprised one bit getting such a brilliant story telling coming from none other than Greg Rucka and perfectly carried in the illustration by J.H. Williams III. More on the art later below.
The story is completely captivating. It just absorbs the reader in for it has a huge number of WOW moments and of course the highly looked upon origin of Batwoman. No Batwoman is nothing like any others in the Bat family and definitely not an ordinary vigilante. She has perfect reasons and motives to serve as the Gotham's new protector.
Rucka's attention to details is remarkable. There is an explanation to what the colors of her suit means, when, how and where does all her tech come from, whats the material her suit is made of, etc. Kate Kane is depicted so beautifully with her life swinging between her emotions, stress, career and issues relating to her sexuality that I gained instant respect and attachment to the character. Greg Rucka has not only presented certain things about our society but also passed on a fully modern moral take on it. I am very very happy that I picked this book up & I recommend this book to everybody out there, not just comic readers but also who never have ready any comic till date! This book is HUGE I tell you!!!
Keep in mind that initially this story has some spinning that you may not fully get but read on and the revelations will take you by surprise I tell you!
And oh by the way - This has one of the best Batman appearances ever - IMO.
Art
I am spellbound by the painted art form of illustration used in this book. I have very rarely seen this kind of art (except for Batwoman New 52) where the panels are simply afloat and do not confine to any particular shape. There are pages where the entire spread is showing you some parts of the past while some small nearly bat shaped floating panels show events occurring in present - How cool is that! I do not have to tell you much as you might probably have already googled the images.
Plus - There is a very cool introduction by in the beginning of the book by a non comic industry figure & super cool Batwoman/ Kate Kane art concepts at the last few pages followed by lovely variant cover gallery at the end!!
Simply eye popping and I do not want to say more - just go and grab this book!!!!
This book truly deserves 5 stars!!
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Reviewed in the United States on October 4, 2012
★★★★★ 5
An Inspiration
After watching a video by one of my favorite youtubers discussing comics and this book, I had to buy it. I have always loved Kate Kane since started Batwoman 52 about a year ago, and was very excited to purchase this graphic novel. My expectations were very high, yet this book still managed to exceed them!
Williams and Rucka are both extremely talented gentlemen. As the introduction points out, they really do convince you that these characters are real. You almost can't believe Kate isn't an actual human being, and you can really imagine meeting her in real life. I also was very (if not more) impressed with the artwork. The compositions are simply stunning, and completely original. I absolutely love how the scenes with Kate as Batwoman are illustrated with lots of detail and fluidity. It really has an eery watercolor effect, with great linework and color. This completely contrasts the scenes where Kate is either out on the town or in her home. Much more graphic, but still delightful. The costumes in this series are also amazing. Both Kate and her foe have some stunning outfits, and are very unique.
Since I had already read Batwoman 52, I knew how everything that was going to go down. A lot of this book was her origin and was very interesting. And although I already knew what was going to happen, it still managed to make me cry. Don't think it won't be exciting or interesting just because you already know her origin, Williams and Rucka really pull you in and keep it very exciting.
Kate Kane is definitely my comic book hero, and is truly an inspiration. If you are just getting into Batwoman or comics, this is a great place to start. Of course you may be disappointed when no other series can compare!
What a shame Batwoman 52 is about to come to an end, this really is as good as it gets people, and I am very sad to see such a creative and talented team leave, but I do understand completely.
I highly recommend this book to Kate Kane, DC and comic book fans everywhere!
Enjoy!
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Reviewed in the United States on September 14, 2013