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Description
S-RMab® CD20 Recombinant Rabbit mAb (SDT-R133)Product Specification Host Rabbit Antigen CD20 Synonyms B lymphocyte antigen CD20, Bp35, Leukocyte surface antigen Leu 16, MS4A1 Immunogen N A Location Cell membrane Accession P11836 Clone Number SDT R133 Antibody Type Rabbit mAb Application WB, IHC P, ICC Reactivity Hu, Ms, Rt Purification Protein A Concentration 0. 25 mg ml Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from
Product Specification
| Host | Rabbit |
| Antigen | CD20 |
| Synonyms | B-lymphocyte antigen CD20, Bp35, Leukocyte surface antigen Leu-16, MS4A1 |
| Immunogen | N/A |
| Location | Cell membrane |
| Accession | P11836 |
| Clone Number | SDT-R133 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | null |
| IHC-P | 1:1000 | null |
| ICC | 1:250 | null |
Background
The CD20 antigen is a membrane-embedded, non-glycosylated phosphoprotein, 33-37 kDa. CD20 functions as a Ca2+-permeable cation channel, involved in the regulation of B-cell activation, proliferation and differentiation. CD20 appears on the surface of the pre-B lymphocyte between the time of light chain rearrangement and expression of intact surface immunoglobulin and is lost just before terminal B-cell differentiation into plasma cells. Surface expression of CD20 on activated B cells is approximately 4-fold greater than that found on resting B cells. CD20 is virtually specific for normal B-cells. A weak expression has been demonstrated in a subpopulation of T-cells, but not in any other cell type [NordiQC].
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Exchange/Return Notes
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