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Description
Mouse SLAMF2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Signaling Lymphocytic Activation Molecule Family, Member 2 (SLAMF2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Signaling Lymphocytic Activation Molecule Family, Member 2 (SLAMF2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Signaling Lymphocytic Activation Molecule Family, Member 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Signaling lymphocyte activation molecule family member 2, also known as SLAMF2, CD48 antigen, or B lymphocyte activation marker (BLAST-1), is a protein encoded by the CD48 gene. It is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF), which includes SLAM (signaling lymphocyte activation molecule) proteins such as CD84, CD150, CD229, and CD244. It is present on the surface of lymphocytes and other immune cells, dendritic cells, and endothelial cells, and is involved in the activation and differentiation pathways of these cells. It was the first B cell-specific differentiation antigen identified in transformed B lymphoblasts. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
Great balls
So far so good. Belgian/pyrenees mix that loves chewing and destroying toys. He’ll have a hard time destroying these. They are light but carry well once thrown outside. Being light they are good inside on very cold days compared to something like a lacrosse ball. Better than Chuck It brand.
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Reviewed in the United States on February 7, 2026
★★★★★ 5
Amazing Buy
These masks are exactly what they say on the package. 10/10 Clears, smooths, and moisturizes your face. Keep on over night, easy to use
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Reviewed in the United States on April 8, 2026
★★★★★ 5
Favorite for dry climate
I live in a dry desert and these are the only moisturizing masks I see instant results. For best results after doing my evening skincare routine I apply the mask one hour before bed. I store them in the fridge so they feel cool and soft on my skin. Once you get the hang of applying them it’s very easy the second time. I sleep with them on and remove them in the morning and my skin is glowing, plump and pores appear smaller. The mask itself is a thick durable jelly not like the typical sheet masks. The price for this is worth it if you wear the mask minimum 2-5 hours once a week
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Reviewed in the United States on November 12, 2025
★★★★★ 5
Youth Serum?
You will not regret trying this! The results are amazing!!! 🤩 After the first use my skin looked healthier, brighter, more even toned and pores were diminished. I look young again. (I’m 57.)
I have neglected my face for a few years after being the kind of girl who never left the house without makeup. I knew I needed some deep repair and thought a mask would help. Lo and behold, my beloved Amazon gave me Biodance! I just used my third mask last night and I’m still excited to peel it off the next morning. My face doesn’t look all dewy like the pictures and video but it’s definitely a huge improvement. I didn’t realize it was that dry. A couple tips-don’t worry when you first apply it if it’s not perfectly positioned. There is wiggle room and it will slide a bit. Handle carefully! It’s slimy & slippery. You can use the eyes and lips (cut outs) for other problem areas and you can tear off the nose as well. It feels cooling the moment you put it on. I had no irritation at all. I took the advice of one reviewer and sat on the couch with my head leaned back for about an hour before I went to bed to let it dry a little. Nice “ME” time. Thanks a zillion Amazon and Biodance! I vow to use a mask weekly from now until the end of time. ❤️
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Reviewed in the United States on December 14, 2025
★★★★★ 5
Cool Product
I love these masks. The are cool and white in color when you put them on and they turn clear when the stuff is absorbed. I was new to a mask that has to be on 3 hours or more, but it was cool. I had very little skin irritation and I have sensitive skin. They are very easy to put on and take off. They have a heavy cooling sensation when you first put them on because the are really wet, but it dissipated over time and was comfortable to sleep in. I use these masks twice a week. I will definitely keep using them. My skin feels softer and more firm in the morning. I just take off the mask and apply a little moisturizer with sunscreen and I'm good for the day.
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Reviewed in the United States on December 13, 2024