Pay in installments of $39.68 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 9 - Aug 14
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse IL-1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 pg/mL). Then dilute to the following concentrations: 200 pg/mL, 100 pg/mL, 50 pg/mL, 25 pg/mL, 12.5 pg/mL, 6.25 pg/mL, 3.125 pg/mL, and 0 pg/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 200 pg/mL standard working solution into the first EP tube and mix thoroughly to make a 100 pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 1 (IL-1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 1 (IL-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 1 ELISA kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Interleukin-1 (IL-1) is a cytokine with a molecular weight of 17.5 kDa. It is produced by various cells, including macrophages, monocytes, and lymphocytes, and participates in a variety of tissue responses. It acts not only locally but also systemically, particularly in large fractures. Within hours of BMP implantation, IL-1 is detected in the blood and can release progenomic genomic DNA (PGE2) from the cell membranes of fibroblasts and osteocytes. IL-1 can also induce rejection of allogeneic bone grafts. Immunosuppressants such as glucocorticoids and cyclosporine are potent inhibitors of IL-1 synthesis and cellular responses to IL-1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 24 reviews
Sort
Product Reviews
★★★★★ 2
Charger portion stopped working twice now
Works well until it doesn't. Was excited to get the phone mount off the car vent but I have gone through one replacement already and now I'm past the point to completely return it. Twice now the charging portion has failed. The first one stopped lighting up completely with in about a week and now this one just constantly flashes and no longer charges after another few weeks. I have tried different cords and outlets in my car with no success. The magnet portion however is strong and does hold the phone well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 15, 2025
★★★★★ 1
Do not buy this junk... it will damage your car!
This junk broke my dash!!! See pictures
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 11, 2025
★★★★★ 5
Perfect for every day use
The Wincar Magnetic Wireless Phone Mount and Charger is a thoughtfully designed accessory tailored for Toyota 4Runner models from 2010 to the present, and it also fits well with other vehicles using a 17mm ball mount base. The package includes a sturdy mount, a 15W magnetic wireless charger, a charging cable, and two steel rings. These steel rings are particularly useful for those who do not own a MagSafe-compatible phone or case, ensuring broader compatibility.
I specifically purchased this charger and holder to use with my existing 17mm ball mount base, which I found challenging to match with most other products—especially for my Lexus GX460. While there are many phone holders on the market, very few offer the dual functionality of both MagSafe mounting and wireless charging in one unit. This all-in-one solution eliminates the need for multiple accessories, but it’s important to note that the charger /mount
require a power connection to operate.
In terms of performance, the Wincar mount and charger deliver impressively. When connected to a reliable USB-C charging source or a USB-C cigarette lighter adapter, the magnetic grip is strong, keeping the phone securely in place even during bumpy rides. The wireless charging is efficient, providing a reasonably fast charge without overheating or lag. The magnetic force is particularly robust, ensuring that the phone remains stable and aligned for optimal charging.
Overall, this mount and charger combination offers a seamless, convenient experience for anyone seeking a reliable phone holder and charger for their vehicle. For my needs, it has proven to be both practical and effective.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 30, 2025
★★★★★ 5
A Solid Mount with Minor Imperfections
This MagSafe mount is a fantastic addition to my 2024 4Runner, offering a nearly factory-fit look. Installation was straightforward, though it required a bit of nerve. I was worried the clips attaching to the AC vent would snap, but with firm, steady pressure, they clicked securely into place without any issue. The result is a very stable platform for my phone.
Functionally, it performs exactly as hoped. It supports fast charging when paired with a capable cigarette port adapter, and the magnetic connection is surprisingly strong. It holds my heavy S25 Ultra securely during daily commutes, and the inclusion of two metal rings for non-MagSafe cases is a thoughtful touch. I haven't tested its limits on a bumpy off-road trail, but for normal driving, it’s perfect.
My only two critiques are minor but worth noting. With the phone in place, it completely blocks the hazard light button. A slight inconvenience. Additionally, I wish the mount's color matched the silver trim around the infotainment system for a more seamless look. Despite these small flaws, it's an excellent, clean mounting solution that I would recommend to other 4Runner owners.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 28, 2025
★★★★★ 5
Five Stars — Finally Got My Charger Back!
Color: Black, Color: Black
I bought this 3-in-1 wireless charging station as a gift for my girlfriend so she would finally stop stealing mine — and let me tell you, it worked. She loves it, her devices love it, and I love not having to play “Where’s my charger?” every night like it’s some kind of relationship scavenger hunt.
Pros:
• MagSafe is strong — Her phone snaps on like it’s reconnecting with its soulmate.
• Charges iPhone, Apple Watch, and AirPods all at once — AKA: no more tangled octopus of cables on the nightstand.
• Looks clean and modern — Her side of the room instantly leveled up in aesthetics.
• Fast charging — She swears her phone charges “faster than her attitude in the morning,” her words not mine.
Cons:
• Now she judges my setup — Apparently I’m the one with the “messy” charger now.
• Might make you buy a second one — Because the moment you try to use hers, she suddenly remembers boundaries.
Overall, great gift, works perfectly, and saved my sanity. Would 100% buy again — but I don’t have to, because she finally stopped borrowing mine!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 19, 2025