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Description
Rat IL-1β/IL-1F2 ELISA KitProduct Specification Usage 1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) 2. High precision liquid dispenser and disposable tip 3. Distilled or deionized water 4. Bottle washer (spray bottle), multi channel plate washer or automatic plate washer 5. 500mL 1. Preparation before the experiment 1. Sample collection and storage Cell culture supernatant:
Product Specification
| Usage |
1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) 2. High precision liquid dispenser and disposable tip 3. Distilled or deionized water 4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer 5. 500mL 1. Preparation before the experiment 1. Sample collection and storage ① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. 2. Reagent Preparation ( Please place all reagents and samples at room temperature before use and let them stand 15 Do repeat hole detection ） ①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Example: Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL ②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. Example: 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. ③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Example: Used 10 Hole, then take 10uL Of 100 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. ④SA-HRP SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL Example: Used 10 Hole, then take 25uL Of 40× +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× ⑤ Color development solution: per well 100uL ⑥ Standard: Dilution buffer for lyophilized standard ( 1× 1000uL , obtaining a concentration of 4000pg/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× 4000pg/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg/mL ）。 2. Operation steps 1. Prepare all necessary reagents and standards. 2. Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; 3. Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; 4. Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ; 5. Suck the liquid in the plate and wash the plate using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3 6. Within each well added 100uL 2 Hour; 7. Repeat th 5 Step washing operation; 8. Within each well added 100uLSA-HRP 20 Minutes. Be careful to avoid light; 9. Repeat th 5 Step washing operation; 10. Within each well added 100uL 5-30 Minutes, pay attention to avoiding light; 11. Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; 12. After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; 13. Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Using computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. Note: The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. 3. Kit parameters 1. Recovery: Different levels were spiked into cell culture medium samples Rat IL-1β/IL-1F2 The recovery rate was determined. The recoveries range from 82-110% , the average recovery was in 94% 。 2. Sensitivity: Rat IL-1β/IL-1F2 The lowest measurable dose ( MDD ) is generally less than 1.8pg/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve. 3. Linearity: 4 High concentrations of different samples were incorporated into Rat IL-1β/IL-1F2 , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. 4. Specificity: This ELISA Method can detect natural and recombinant Rat IL-1β/IL-1F2 Egg whites. The following factors were mixed with diluent ( 1× ) configured to 50ng/mL Concentration to be detected with rats IL-1β Cross-reactivity of. Will 50ng/mL Interference factors incorporated into the intermediate range of recombinant rats IL-1β In the control article, to detect the effect on rats IL-1β Of interference. No significant cross-reactivity or interference was observed.
4. Analysis of frequently asked questions 1. Whiteboard (no color appears after color rendering is complete)
2. Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher)
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| Species Reactivity | Rat | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-rat IL-1beta/IL-1F2 antibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the IL-1beta/IL-1F2 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Synonym | catabolin, IL1 beta, IL-1 beta, IL-1, IL1B, IL-1b, IL1-beta, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1 beta, Rat interleukin 1β Elisa kit | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Interleukin -1 （ IL-1 ) Protein family members include classical IL-1 And IL-1 。 IL-1 Receptor antagonists ( IL-1RA ）、 IL-18 、 IL-33 、 IL-1F5-F10 、 IL-1 And IL-1 Have the same cell surface binding receptors and form the same biological function. Unstimulated cells in healthy people do not produce IL-1 The only exceptions are skin keratinocytes, some epithelial cells, and some cells of the central nervous system. But in response to inflammatory agents, infection, or microbial endotoxin, IL-1 Expression in macrophages and other types of cells increased dramatically. IL-1 In immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, GH/IGF-1 Plays an important role in the physiological process of. Multiple diseases and IL-1 Dysregulated expression or delayed expression associated, including diseases related to sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury and senile degeneration. IL-1 And IL-1 Are structurally related polypeptides, at the amino acid level about 25% Homology of. Both are first synthesized into 31kDa Precursor, then cleaved to approximately 17.5kDa Of the mature protein. By Caspase-1/ICE Right IL-1 Cleavage of precursors is a critical step in the inflammatory response. Despite IL-1 And IL-1 Neither contain a typical hydrophobic signal peptide, but there is evidence that they can be secreted extracellularly through non-classical pathways. Unprocessed IL-1 Parts may be displayed on the cell membrane and may retain biological activity. With IL-1 The precursors are different, IL-1 Precursors vs. mature IL-1 Compared to having little or no biological activity. IL-1 Precursors vs. mature IL-1 All transported extracellularly. IL-1 And IL-1 Via immunoglobulin superfamily receptors and IL-1RA Combine to exert its effect. 80kDa Of I Type transmembrane receptor ( IL-1RI ) expressed in a variety of cells, including T Cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes. 68kDa Of II Type transmembrane receptor ( IL-1RII ) in B Cells, neutrophils and bone marrow cells. IL-1RI And IL-1RII The extracellular region of 28% Homology of. But there are significant differences in other areas: II The intracellular domain of type receptor is only 29 Amino acids, and I The intracellular domain of type receptor is 213 Amino acids. IL-1RII It seems to be right IL-1 The signal does not respond, its function is to decoy the receptor and thus weaken IL-1 The role of. IL-1 Receptor ligand protein ( IL-1RAcP ) with IL-1RI The interaction is IL-1RI Necessary for signaling. IL-1RA Is a secreted molecule, is IL-1 Competitive inhibitors. Soluble IL-1RI And IL-1RII It is present in human plasma, joint fluid, and conditioned media of several cell lines. Moreover, vaccinia virus encodes similar to soluble IL-1RII Of IL-1 Binding proteins. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Unopened kit, store at 2–8 °C | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Test Range | 62.5pg/mL-4000pg/mL |
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4.5 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
Solid, grippy, and they don’t roll away!
Style: 10 Pound, Pair
I bought these because I’m finally trying to build a consistent home workout routine and didn't want to drop a fortune on name-brand weights. I’ve been using this 10lb pair for about a month now for everything from overhead presses to lunges, and honestly, they’re exactly what I was looking for.
The Pros:
• That Neoprene Grip: The coating is fantastic. Even when my hands get pretty sweaty halfway through a workout, I don’t feel like I’m going to drop them. It’s a much "softer" feel than cold metal or hard plastic.
• The Hex Shape: This is a small detail that makes a huge difference. Since the ends are hexagonal, they don't roll across the floor when I set them down between sets. They also stay put if I’m using them for "renegade rows" or floor work.
• Compact Size: They aren’t bulky. I can easily shove them under my bed or in the corner of the closet when I’m done.
• No Weird Odor: I was worried they might smell like a tire factory (which happens with some rubber-coated weights), but these had almost no scent right out of the box.
The Cons:
• Handle Diameter: The handles are a little on the thicker side. I have medium-sized hands and it’s fine, but if you have very small hands, they might feel a bit chunky to wrap your fingers all the way around.
• Color Scuffs: The navy blue looks great, but if you clank them together or drop them on concrete, the coating can scuff or show dust pretty easily.
The Verdict:
If you just need a reliable set of weights for HIIT, Pilates, or general strength training, these are a no-brainer. You're basically getting gym-quality equipment for a fraction of the price. I’ll probably be back for the 15lb set in a few months!
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Reviewed in the United States on January 27, 2026
★★★★★ 5
Simple, Comfortable, and Exactly What You Expect
Style: Rack with 3 Pairs (5, 10, and 15 Pound)
These are pretty much exactly what you want from a set of dumbbells — simple, functional, and no nonsense. The neoprene coating gives them a comfortable grip and keeps them from feeling slick in your hands, especially during longer workouts or if your hands get sweaty.
The coating also helps keep them from banging up floors or making as much noise compared to bare metal weights, which is nice if you’re working out at home.
Weight accuracy seems fine for general fitness use, and they’ve worked great for things like accessory movements, shoulder work, lighter presses, rehab-style exercises, and general muscle toning. The shape also helps keep them from rolling around everywhere when set down.
They’re not fancy, and they’re not trying to be — they’re just dependable dumbbells that do what they’re supposed to do at a fair price.
If you want straightforward hand weights for home workouts without overthinking it, these are a solid buy.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Reliable, Comfortable Dumbbells I Actually Use Every Day
Style: 12 Pound, Pair, Style: 12 Pound, Pair
I’ve been using the Amazon Basics Neoprene Dumbbell Hand Weights daily, and they’ve honestly been perfect for my routine. Whether I’m doing a quick morning workout or adding resistance to a longer session, these have held up really well.
The neoprene coating makes a big difference—it provides a comfortable, non-slip grip, even during longer workouts or when my hands get a little sweaty. They’re also easy to clean and don’t have that strong rubber smell some weights can have.
I really appreciate the hex shape too. They stay put when I set them down (no rolling across the floor), which makes switching between exercises quick and frustration-free. The weight feels evenly distributed and well-balanced, so movements feel controlled and smooth.
Overall, these are simple, no-frills dumbbells that just work. If you’re looking for reliable hand weights for everyday use—whether you’re a beginner or just need something dependable for home workouts—these are a great choice.
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Reviewed in the United States on April 14, 2026
★★★★★ 5
Great quality and perfect for home workouts!
Style: Rack with 3 Pairs (3, 5, and 8 Pound)
I’m really happy with this dumbbell set. The weights are clearly labeled (3 lb, 5 lb, and 8 lb), which makes it super convenient to switch between exercises. The neoprene coating feels comfortable in hand and provides a good grip, even during longer workouts.
The storage stand is a big plus—it keeps everything organized and saves space, which is perfect for a small home gym. The colors are also nice and make it easy to quickly grab the weight you need.
Overall, this is a great set for beginners or anyone looking to add light strength training to their routine. Highly recommend!
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Reviewed in the United States on April 13, 2026
★★★★★ 5
I’m A PT AND USING THEM IN MY CLINIC. PLS READ HONEST AND THOROUGH REVIEW.
Style: 15 Pound, Pair
I have been a PHYSICAL THERAPIST FOR 30 YEARS and I BOUGHT THESE FOR MY CLINIC. THESE WEIGHTS ARE COVERED IN A GRIPPING MATERIAL, NEOPRENE, MAKING IT COMFORTABLE and EASIER TO USE, ALLOWS YOU TO NOT LOSE YOUR GRIP, and IT IMPROVES THE SAFETY OF THE USER. I WILL BE BUYING THE 12&20 lb sets! THE VALUE FOR YOUR MONEY IS WONDERFUL!! AMAZON BASICS REALLY CAME THROUGH. It DID NOT INCLUDE AN INSTRUCTION GUIDE TO USE WEIGHTS SAFELY AND EFFECTIVELY. STELLAR FORM IS ALWAYS ESSENTIAL.
MY WEIGHTS WENT UP TO 10 POUNDS, SO I DEFINITELY NEEDED ADDITIONAL WEIGHTS for those who can SAFELY TRANSITION . I MAINLY WORK AS A MANUAL THERAPIST …USING ONLY MY HANDS, AND MY BRAIN. TO DIAGNOSE AND TREAT ACCORDINGLY.
(**ANYONE WHO HAS BEEN TO PT KNOWS THIS KIND OF ONE ON ONE TREATMENT IS UNHEARD OF**)
I IMPLEMENTED MYOFASCIAL RELEASE, TRIGGER POINT THERAPY, DEEP TISSUE MASSAGE, AND APPROPRIATE STRETCHING AND EXERCISE . ONCE PATIENTS WERE ABLE, I INITIATED THERAPEUTIC EXERCISE. I WAS GETTING SO MANY REFERRALS THAT I MOVED FROM MY SMALL SPACE AND GOT A LARGER SPACE THAT LOOKS LIKE A TYPICAL PT CLINIC. I STILL EMPLOY ONE ON ONE TREATMENT, NO JUGGLING SEVERAL PATIENTS IN AN HOUR. I HAVE HIRED HELP TO ASSIST PATIENTS WITH EXERCISE AND STRETCHING. I PURCHASED TWO ADDITIONAL SETS ITHE SET IT I DID TO FIRST CHECK THEM OUT. I ADORE THESE AND HAVE SINCE ORDERED OTHER SETS. I LOVE TO WORKOUT AND I NEEDED TO USE THESE WEIGHTS MYSELF!! THERE ARE ALSO PATIENTS OF MINE WHO WILL PROGRESS TO THESE AS WELL. I AM SUPER IMPRESSED WITH THESE WEIGHTS AND CANNOT WAIT TO RECEIVE MY TWO OTHER SETS!! YOU WILL NOT BE DISAPPOINTED.
EVEN IF YOU SWEAT, YOU ARE STILL ABLE TO HOLD ONTO THESE WEIGHTS COMFORTABLY AND SAFELY. SAFETY IS KEY, ALWAYS. I WAS EXTREMELY DELIGHTED THAT THEY ARE SOLD AS A SET BECAUSE MANY ARE SOLD SEPARATELY. THEY FIT PROPERLY ON A UNIVERSAL WEIGHT STAND, SO THEY ARE VERY EASY TO STORE. I CANT WAIT TO GET MY OTHER SETS! IT IS REALLY SAFE AND COMFORTABLE TO USE THESE WEIGHTS.
THEY DID NOT GIVE OFF AN ODD ODOR, which is A HUGE PLUS because SOME OTHER BRANDS SMELL HEAVILY OF PLASTIC OR A CHEMICAL TYPE ODOR. THERE WAS NO ODOR AT ALL. THIS IS A HUGE PLUS. I AM COMFORTABLE SAYING THAT THESE WEIGHTS WILL LAST A LONG TIME BECAUSE OF HOW WELL THEY ARE MADE. YOU CANNOT BEAT THE PRICE EITHER!!
THESE ARE ABSOLUTELY APPROPRIATE FOR PROFESSIONAL USE, AND, THEY ARE MEANT FOR PERSONAL USE AS WELL. SOMETIMES YOU JUST CANNOT GET TO THE GYM SO THIS IS WHERE THEY ARE KEY. HAND WEIGHTS HAVE THE ABILITY TO EXERCISE EACH MUSCLE OF THE TORSO AND ARMS. THERE ARE SOME EXCELLENT WEIGHT CHARTS OUT THERE TO SHOW YOU HOW TO DO THAT. IF YOU ARE GOING TO PHYSICAL THERAPY, THEN YOUR THERAPIST CAN INSTRUCT YOU AND KEEP YOU IN GOOD FORM. YOU COULD POSSIBLY HURT YOURSELF IF YOU WORKOUT INCORRECTLY. AS FAR AS THE LOWER EXTREMITIES, YOU CAN DO GROSS MOTOR EXERCISES BY HOLDING THESE WEIGHTS TO GET YOUR LEG MUSCLES BY DOING SQUATS, WALKING LUNGES, TOE RAISES, AND MORE. I DONT BELIEVE THAT MANY PEOPLE REALIZE JUST HOW MUCH YOU CAN DO FULL BODY EXERCISES WITH JUST TWO HAND WEIGHTS.
IN CONCLUSION , THESE ARE EXCELLENT WEIGHTS THAT I WOULD RECOMMEND TO MY PATIENTS OR FRIENDS. THIS IS A HIGH QUALITY PRODUCT THAT WONT BREAK YOUR PIGGY BANK. I HIGHLY RECOMMEND VERY HIGHLY!. BUY WITHOUT RESERVATION!! A+++
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Reviewed in the United States on March 23, 2025
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