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Description
Human myonectin ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are added sequentially to microwells pre-coated with a myonectin capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the myonectin content in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human myonectin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Myonectin is primarily expressed and secreted by skeletal muscle. It is a nutrient-responsive metabolic regulator that responds to changes in cellular energy status induced by glucose or fatty acid flux. Many secreted proteins involved in metabolism (such as adipose tissue proteins, leptin, resistin, and RBP) and the signaling pathways they regulate in tissues are known to be dysregulated in obesity. The reduced expression and circulating levels of carnosine in obesity may represent another component of the complex metabolic circuitry triggered by excess caloric intake. Although exercise has long been known to have profound positive effects on systemic insulin sensitivity and energy balance, the underlying mechanisms remain incompletely understood. Voluntary exercise significantly increases the expression and circulating levels of myonectin to facilitate cellular fatty acid uptake, which is one of the foundations for the beneficial effects of physical exercise. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.9 ★★★★★
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Product Reviews
★★★★★ 4
Quality meets style
Color: White, Size: Large, Color: White, Size: Large
This is an Oxford button-down dress shirt that is a great example of quality craftsmanship paired with classic style. The 50% polyester and 50% cotton fabric has a nice substantial weight to it that feels durable without being too heavy, making it perfect for a polished summer look. The detailed design around the collar adds a subtle touch of casual sophistication, and the chest pocket keeps it practical and versatile.
The construction is impressive, with securely attached buttons that reinforce the overall sense of durability. I also appreciated the inclusion of two extra button which is a thoughtful and useful addition.
The size large did run a bit snug so I’ll be ordering the next size up. This is a well-made shirt, of a quality-feel material. This brand continues to deliver on both quality and style, and the price feels more than fair for what you’re getting. Overall, a very nice summer dress shirt that I’d gladly recommend.
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Reviewed in the United States on March 30, 2026
★★★★★ 3
Good quality but need to be aware.
Color: White, Size: XX-Large
Good quality but know Sizes run small so size up when ordering if you are a US customer. Also chest is tight even when sizing up.
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Reviewed in the United States on June 2, 2026
★★★★★ 5
Upsize
Color: Pink, Size: XX-Large
Knowing to upsize before I bought the shirt was a good thing to know. Otherwise, it is a very nice shirt
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Reviewed in the United States on April 30, 2026
★★★★★ 5
As I expected, Manly
Color: Blue, Size: Medium
this is a beautiful well made shirt
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Reviewed in the United States on April 8, 2026
★★★★★ 5
No flaws, not high end
Color: Pink, Size: Medium
I nabbed this for my in shape 14 year old son who had an upcoming wrestling banquet. It fit very true to size for him however as a low-mid grade dress shirt it had little flex or mobility across the chest and that’s ok, this is an entry level dress shirt and perfect for this and similar events.
The color was vibrant and well saturated, and the fabric itself was thin enough for some transparency but thick enough to be considered medium weight. It would not be a cooling dress shirt.
It did not have a soft hand, rather a sturdy rougher feel akin to a dress shirt a restaurant server would wear and laundry frequently which is also ok because it is exactly what a polo in this range should be. If you are looking for a flexible chest and soft touch you will need a better fabric blend and you pay a lot more for that.
The buttons were nice and collar looked good.
I would recommend this for any average event or occasion or even for work. If you are looking to impress on an interview or a date or during high heat you might need to look elsewhere.
For what it is it’s perfect. Buyer and shirt need to remember this and keep it in its shirt lane.
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Reviewed in the United States on April 18, 2026