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Description
Human IL-12 p70 ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human IL 12 p70 antibodies were pre coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL 12 p70 present in the sample binds to the solid phase antibody
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-12 p70 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL-12 p70 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | Human interleukin 12p70 detection kit, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NKSF1, p35 | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Interleukin 12 p70 (IL-12), also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine consisting of a 40kDa (p40) subunit and a 35kDa (p35) subunit. Human p40 may circulate as a monomer, a homodimer, or a complex that binds to p35 to form IL-12 or binds to p19 to form IL-23. Both homodimers p40 and IL-23 can bind to IL-12R as non-signal antagonists. Alternatively, p40 can also bind to Rβ1, activating microglia and macrophages. The IL-12 p40 subunit shares with another heterodimeric cytokine, IL-23, with similar and distinct biological activity from IL-12. Cells known to produce IL-12 include macrophages and dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, microglia, CD8 + DCs (mouse cells only), and non-germinal center (CD38-CD44 +) B cells (human cells only). Functional IL-12 was shown to enhance cytotoxic activity and induce interferon gamma (IFN-gamma) production within NK cells, T cells, and dendritic epidermal T cells. IL12 has also been reported to induce IFN-γ production by macrophages. IL-12, together with other members of the IL-12 family, IL-23 and IL-27, promotes the development of CD4+Th1 immune response. In response to infection, IL-27 is first released, promoting the transition from Th0 to Th0/1. IL-12 is subsequently produced, resulting in Th1 effector cells. IL-12 combines with IL-18 to generate Th1 memory cells from effector cells, which are subsequently activated by IL-23. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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4.3 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 5
Great Tray For Storing My Everyday Carries
Size: 8.5" x 5.8" x 1.9", Color: Black
My everyday carry items (wallet, keys, etc.), used to just get thrown around wherever when I'd get home, but this little tray makes a great, defined, space for me to keep those things when I don't need them on me. The soft lining reduces the obnoxious sound of throwing keys on a hard surface, and of course keeps me from scratching up a surface. The build feels quality and I haven't seen any damage to it yet. Also the all black version with gold buckle looks quite good and adds a subtle touch of luxury. Absolute recommend for the price.
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Reviewed in the United States on April 15, 2026
★★★★★ 5
Just the right size to empty your pockets.
Size: 8.5" x 5.8" x 1.9", Color: Orange
Perfect size to empty my pockets when I get home. Fits my wallet, keys, AirPods, etc. excellent quality.
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Reviewed in the United States on March 21, 2026
★★★★★ 5
Nice and classy
Size: 8.5" x 5.8" x 1.9", Color: Black
Bought this for my husband for Christmas and he loved it. Super classy elegant well made box. Perfect for bedside nightstand for your watch and rings.
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Reviewed in the United States on December 4, 2025
★★★★★ 5
Nice looking and durable
Size: 8.5" x 5.8" x 1.9", Color: Black
I bought this for my little nephew to put his rocks in and out and to keep them in. It’s perfect and durable.
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Reviewed in the United States on January 12, 2026
★★★★★ 5
Perfect size
Size: 8.5" x 5.8" x 1.9", Color: Black, Size: 8.5" x 5.8" x 1.9", Color: Black
Love the look of leather and metal together.
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Reviewed in the United States on April 30, 2026
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