SKU: 29306481481

Rat IL-2 High Sensitivity ELISA Kit

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Description

Rat IL-2 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti rat IL 2 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 2 in the sample binds to the solid phase antibody. After

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti rat IL-2 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-2 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-2 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IL-2 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.71 pg/ml

Detection Range: 12.5-800 pg/ml

Recovery Range: 95-119%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 2 (IL-2) is a 15 kDa glycoprotein. In humans, it is encoded by a single gene located in the q26 – 28 region of chromosome 4. It is a class of signaling molecules in the immune system, regulating the immune function of white blood cells (leukocytes, usually lymphocytes)Activity of. IL-2 is the body's natural response to microbial infection, distinguishing foreign and self antigens. IL-2 functions by interacting with the IL-2 receptor, which is composed of three chains, namely α Chain (CD25), β Chain (CD122) and γ Chain (CD132). Monitoring the content of IL-2 in serum provides a more detailed perspective for a variety of pathological conditions, including cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

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SKU: 29306481481

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MarsBars
West Palm Beach, US
★★★★★ 5
It works well
Size: Large
Works great and last long .
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Reviewed in the United States on May 11, 2026
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Kwhitt
Omaha, US
★★★★★ 4
Great alternative to a washcloth
Size: Large
Love the exfoliating texture! I have them on automatic delivery. I have ditched washcloths and loofahs for these. Hold soap well. Dry quickly. Hang from the tag. Last the whole month without falling apart. A little expensive but I think they are worth it.
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Reviewed in the United States on May 17, 2025
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CynthiaP
Belleville, US
★★★★★ 5
Love these Exfoliating Sponges
Size: Large
These sponges do a great job. Love the colors. They are not to rough on the skin. You do not have to press down to hard to get smooth skin. They are well worth buying.
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Reviewed in the United States on February 12, 2026
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TexasRee
Port Orchard, US
★★★★★ 5
Excellent for rough dry skin
Size: Large
I love this product. I use these exfoliating scrubs with the Tree Hut Vitamin C Shea Sugar Scrub, . My legs had been looking really dry and rough and really horrible. I had to figure out what to do about it. This product with the scrub really did the job. In fact a friend was over--i was in the recliner and had my legs and she asked if I had hose on because my legs were so smooth! (Her birthday is next month so I will order these products for her!)
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Reviewed in the United States on November 15, 2025
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Jennifer M.
Port Orchard, US
★★★★★ 5
Excellent!
Size: Large
Works very well!! Love it!!
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Reviewed in the United States on April 21, 2026

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