SKU: 29670255549

Human FGF basic ELISA Kit

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Description

Human FGF basic ELISA KitProduct Specification Synonym basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF 2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF 2; heparin binding growth factor 2; Prostatropin Description Testing Principle This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti human FGF basic antibody is pre coated on a high affinity plate. Standards, test samples, and biotinylated

Product Specification

Synonym basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin
Description

Testing Principle

This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human FGF basic antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the FGF basic present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).

 

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity100ul

Kit composition:

96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification

Sensitivity:   0.089-0.740 pg / mL

Detection range:   15.6 - 1000 pg/mL

Recovery rate:    94-119%

Storage: 2-8 ° C

The standard curve:

Background: FGF acidic

FGF basic, also known as FGF-2(fibroblast growth factor-2) or HBGF-2(heparin-binding growth factor-2), is the most intensively studied of the 22 mitogenic proteins in the FGF family.FGF family members share 35-60% amino acids (aa), but only acidic and alkaline FGF lack signal peptides,which are secreted by another pathway. The 18 kDa FGF basic subtype can be found in the cytoplasm and nucleus and is also secretory.It may be present in storage tanks of heparin sulphate proteoglycans (HSPG) inside or on the cell surface.Transcription from alternate initiation sites produces a 21-23 kDA form, found only in the nucleus.

Human FGF basic sequences at 18 kDa had aa homology of 97% and 99%, respectively, with mouse/rat and cattle/sheep FGF basic sequences. However, the destruction of basic FGF genes in mice resulted in relatively mild cardiovascular, skeletal, and neurophenotypes, suggesting that other members of the FGF family compensated for this.Transgenic basal overexpression mainly affects bone development and mineralization.

Four FGF tyrosine kinase receptors (FGF R) and their splicing variants showed differential binding of FGF.FGF basic preferentially binds FGF R1c and 2C with picoluminal affinity.FGF basic also has a number of other binding partners that can fine-tune FGF basic activity depending on their location and number. These include heparin, integrin V 3, soluble FGF R1, FGF binding protein, free gangliosides, platelet-reactive protein, pentammeric protein 3, fibrinogen, 2-macroglobulin, platelet-derived growth factor and platelet factor-4, all of which bind in nanomolar affinity. These molecules can act as co-receptors or adhesion chaperones of the cells, bait or reservoir in the extracellular matrix, while scavengers or chaperones can act as free proteins. FGF basic binding to cell surface HSPG is particularly critical and is necessary for binding, dimerization, and activation of FGF R.

FGF basic regulates normal processes such as angiogenesis, wound healing, tissue repair, learning and memory, and embryonic development and differentiation of the heart, bone, and brain.It is upregitated by inflammatory mediators such as TNF-α, IL-1β, IL-2, PDGF and nitric oxide.Many human tumors express basic FGF, which may be related to tumor angiogenesis.

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SKU: 29670255549

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Reviewed in the United States on October 23, 2024
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Brie
Alexandria, US
★★★★★ 5
great ball!
Size: Medium (2.5"), Style: Fetch Pack 2
My pitbull LOVES these. She is a power chewer and tough on balls and toys and tends to go through them insanely quick. She is still on her first ball out of this pack and its been at least two months. It is a little small for her mouth but she doesn’t seem to mind, as it has quickly become one of her favorites. Not a loud ball and it doesn’t usually make that wet chewy noise when shes going to town on it which is huge for me😂😂 Safe for my pittie so i’d imagine a smaller dog would be just fine.
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Reviewed in the United States on April 21, 2026
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Amazon Customer
Grantham, US
★★★★★ 5
Border Collie Approved: Easy to Clean, Durable, and Great Bounce
Size: Medium (2.5"), Style: Fetch Pack 2
These are the best balls out there for dogs. These fit great in the chuck-it ball launcher, which we need to wear out my border collie. They fit her mouth properly and, most importantly, do not absorb all the spit or water after a good rain. They are quick to clean off and have good weight so you can let them fly! Furthermore, they bounce well, which is perfect for my dog that likes to catch them on a bounce. The ball is a strong rubber, so should not be easy to chew up. I've used these for years, and the only time I've had to replace them is when one gets lost in the hay field. I highly suggest these as replacements for the common tennis balls.
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Reviewed in the United States on July 19, 2025
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maxine
Draper, US
★★★★★ 4
Heavy duty.
Size: Medium (2.5"), Style: Fetch Pack 2
My dog was not interested, but it’s a good product
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Reviewed in the United States on April 11, 2026
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M Denise
Omaha, US
★★★★★ 5
Treats and plenty of ball throwing with these doggie toy
Size: Medium (2.5"), Style: Fetch Pack 1
I have actually purchased these twice already. I love the one that glows in the dark but it still got lost in my yard lol. My dog is a big breed half pitt/Labrador so she did chew through one of them already. I play ball with her every single day but I DO have to take them away from her because she will destroy them. They are great to add in treats as well, that was one of the my favorite things about them as well. They do last just if you have an over zealous chewer do not let them keep them for long periods of time. They are a good value for the price. Buy them your dog will love it!
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