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Description
Human GLRX3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a glutaredoxin 3 (GLRX3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of glutaredoxin 3 (GLRX3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Glutaredoxin 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glutaredoxin 3 (GLRX3), also known as thioredoxin-like protein 2 (TXNL2), is a protein encoded by the GLRX3 gene. This gene encodes a member of the glutaredoxin family. The encoded protein binds to and regulates protein kinase C theta. It may also inhibit apoptosis and play a role in cell growth, and its expression may be a marker for cancer. Its pseudogene is located on the short arms of chromosomes 6 and 9. Alternative splice transcript variants of this gene have been observed. Together with BOLA2, it functions as a cytosolic iron-sulfur (Fe-S) cluster assembly factor, promoting the insertion of [2Fe-2S] clusters into a subset of cytosolic proteins. It acts as a key negative regulator of cardiac hypertrophy and a positive inotropic regulator. It is required for hemoglobin maturation. It does not possess any thyroredoxin activity because it lacks a conserved motif required for catalytic activity. It has been shown to interact with PRKCQ. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.2 ★★★★★
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Product Reviews
★★★★★ 5
High quality in every way!
Color: Blush, Size: Full/Queen
Very soft and beautiful! Nothing skimpy about this one! Very pleased.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Light Weight but Not Hot
Color: Dark Grey, Size: King
Ok... I absolutely love this comforter. In fact, I use it on three different beds. The colors are perfect. The material is soft. It's extremely light weight, but it keeps you warm in the winter and does not overheat you in the summer. I have this in King and Queen, and the sizing has been good for both. I have thrown it in my washing machine multiple times, and it holds up well. Great quality, low price.
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Reviewed in the United States on December 3, 2025
★★★★★ 3
Navy is pretty, but doesn’t feel like a great quality set.
Color: Navy Blue, Size: King
I purchased the Navy. The color is great and the pleated pattern is a nice touch. My daughter has the white and it’s very soft. The navy is not, though. So that was disappointing. It’s also not breathable. It’s not thick, but just not breathable. I actually get pretty warm at night with this one when I don’t normally with any other blankets. It also seems on the small side. We have a king and always buy king, this just feels a little smaller. Doesn’t feel high quality. I would not buy again.
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Reviewed in the United States on October 11, 2025
★★★★★ 5
Very Elegant!!
Color: Bright White, Size: Full/Queen
Beautiful comforter set! its super fluffy light weight while still cozy, gives a clean expensive look. It arrives wrinkled up from being packaged, i never steamed mine but you could do that if you want a crisp look. I love the quality and looks more expensive then it was, highly recommend!
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Reviewed in the United States on May 20, 2026
★★★★★ 5
Excellent buy!
Color: Blush, Size: Twin/Twin XL
I LOVE this set! It is perfect all year long! I fold it down in the summer to just cover my feet. In the Fall it’s perfect weight alone and in the colder months I use a blanket underneath! Everyone says it’s Beautiful! The color stays true wash after wash! It retains its shape well also! The pillow case is exactly like the cover! It also retains color & shape! It’s soft and you can just curl up in it! The quality is just as good as some other very expensive brands!
This is great value for the money!
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Reviewed in the United States on September 1, 2025
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