SKU: 31848027119

UniOne® TR-FRET Human IL3/IL3R Binding Kit

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Description

UniOne® TR-FRET Human IL3/IL3R Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the interaction between IL3 and IL3R. Thismethod enables simple and rapid high throughput screening of inhibitors andblocking antibodies. As shown, the interaction between IL3 and IL3R isdetected using a Eu labeled Anti Tag1 Antibody (TR FRET donor) and anAcceptor labeled IL3 protein (TR FRET

Product Specification


Host Human
Stability & Storage

-80℃

Background

This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between IL3 and IL3R. Thismethod enables simple and rapid high-throughput screening of inhibitors andblocking antibodies.

As shown, the interaction between IL3 and IL3R isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled IL3 protein (TR-FRET acceptor). The binding of IL3 to IL3Rbrings the donor and acceptor antibodies into proximity. Excitation of thedonor antibody triggers Fluorescence Resonance Energy Transfer (FRET) to theacceptor antibody, resulting in a specific emission signal at 665nm. Thisspecific signal is proportional to the degree of interaction between IL3 andIL3R. This homogeneous assay is simple to perform and requires no washingsteps.

 


Components

Table 1. Kit Components and Storage Conditions

Component

Concentration

100 TESTS

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

IL3-01A protein

50×

10μL

50μL

250μL

1000μL

-80°C

Tag1-IL3R protein

100×

10μL

40μL

200μL

800μL

-80°C

Anti-Tag1 Eu dye antibody

50×

10μL

50μL

250μL

1000μL

-80°C

Detection buffer

10×

400μL

2mL

10mL

40mL

-80°C

*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.

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Exchange/Return Notes
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SKU: 31848027119

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4.9 ★★★★★
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Product Reviews
E
Verified Purchase
E
Dallas, US
★★★★★ 1
Don't buy AppleCare from Amazon, won't "attach" to your device automatically
Style Name: MacBook Pro 14" (M4), Configuration: 3 years
As another reviewer said, Amazon should not be allowed to sell digital items like these and fail to deliver them. That's called a scam. I have yet to receive my AppleCare agreement, but Amazon was more than glad to charge my Amex right away. Purchasing AppleCare from Amazon will only get you to a download of a PDF of the general terms and conditions, from your "Software Library", but it is not YOUR actual user agreement, and will not register on your device. Amazon will not help either, they will refer you to Apple support, and Apple will tell you that without an Agreement Number, they can't apply your AppleCare to your device. Since the PDF Amazon directs you to is a simple General Terms and Conditions document, there is no Agreement Number. In addition, the purchase invoice doesn't show this Agreement Number nor the device's Serial Number (which Amazon claims is recorded with the purchase... it's not) Save yourself the headache, and buy AppleCare direct from Apple, it will be about $60 more, but the "savings" is not worth dealing with this Amazon stuff. Amazon will also refuse to process a refund, stating that "only Apple can do that". Guess Amazon is telling you to contact your credit card and initiate a chargeback for services not received as paid.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 27, 2024
T
Verified Purchase
Telic
Fort Morgan, US
★★★★★ 5
Was skeptical but it worked out of the box
Style Name: MacBook Air 13" (M4), Configuration: 3 years, Style Name: MacBook Air 13" (M4), Configuration: 3 years
Was skeptical at first but decided to pull the plug and get the m4 laptop with 3 years of apple care and it works. Haven't even opened an email or anything, laptop was still fresh out of the box. When I remembered I bought apple care and decided to check. Applied instantly as far as I know once I linked my apple account on the first boot up with internet! Good stuff
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 4, 2026
H
Verified Purchase
hugo
Phoenix, US
★★★★★ 1
DO NOT PURCHASE WITH YOUR MACBOOK OR OTHER APPLE DEVICES
Style Name: MacBook Air 13" (M4), Configuration: 3 years, Style Name: MacBook Air 13" (M4), Configuration: 3 years
I bought this along with my MacBook. And here is why you SHOULDN'T: 1. They NEVER sent me an AppleCare+ Agreement. You will only be able to download a General Terms & Conditions file. 2. I bought: AppleCare+ for 13-inch MacBook Air (M4) (3 years) and in the General Terms & Conditions that I downloaded, looking for my AppleCare+ Agreement #, it NEVER mentioned Macbook Air as a covered device. It was meant for other smaller devices, which is why it's cheaper. 3. Like I mentioned, since I never received an agreement, I never got an agreement #. 4. Since this AppleCare wasn't actually meant for a MacBook, it didn't connect automatically to my computer. So when I went to talk to Apple and Amazon, without an Agreement # you can do ANYTHING. If you purchase it, read through your General Terms and Conditions and find an excuse to ask for a FULL REFUND. Since this didn't cover my computer, I was thankfully able to get one, but it was a pain trying to talk to customer support and apple.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 27, 2025
A
Verified Purchase
Amazon Customer
New York, US
★★★★★ 5
It worked! Apple Care was added to my device when I received it!
Style Name: MacBook Pro 14" (M4 Pro/M4 Max), Configuration: 3 years
I purchased an MacBook and AppleCare+ at the same time. A day/two after the purchase I received an email from Amazon that the AppleCare+ was in software and go to digital download. When I downloaded it was just the agreement. Then I started reading all the negative reviews on nightmare experience with Amazon and AppleCare+ not being added. I was very nervous and mentally prepare for the nightmare that lie ahead which having to call Amazon multiple times and then having to call Apple multiple times. To my surprise, right after Amazon delivered the MacBook (I got notification your item was delivered) I got another email from Amazon that AppleCare+ was added the laptop with agreement number and serial number of the laptop I received. I checked Apple's coverage site and sure enough Apple site showed AppleCare+ coverage tied the MacBook. I didn't even open the box or turn it on and see the AppleCare+ coverage. Hopefully this gives some insight to future buyers of AppleCare+ from Amazon!!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 25, 2024
C
Verified Purchase
C. Wong
Port Orchard, US
★★★★★ 1
DO NOT PURCHASE APPLECARE+ THROUGH AMAZON (Please read before making a decision)
Style Name: MacBook Air 13" (M4), Configuration: 3 years
I rarely write reviews like this, but DO NOT PURCHASE APPLECARE+ THROUGH AMAZON. Just over a month ago, I purchased an Apple MacBook Air (m4) with AppleCare+. The AppleCare+ wasn't populating on the laptop, so after calls with Apple Support and Apple Agreement Administration, they found that AMAZON sent them the approved agreement with the device serial number then abruptly canceled it the following day. This happened over a month ago, and I was not notified nor have I received a refund. I would not have known it was canceled if not for Apple Support and Apple Agreement Administration. The only recourse is to receive a refund from Amazon--which is NOT automatic, I am fighting for this--and purchase AppleCare+ directly through Apple. Had all this taken place after the 60-days purchasing the MacBook Air (I'm currently one week short of this deadline), then I would be SOL on being able to cover my device. PURCHASE AT YOUR OWN DISCRETION/RISK AND CHECK THAT APPLECARE+ IS APPROVED AND ACTIVE ON YOUR DEVICE. Otherwise, play it safe and activate directly through Apple.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 28, 2025

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