Human/Mouse/Rat PGE2 Competitive ELISA Kit

Detection Principle:

This kit uses solid-phase competitive enzyme-linked immunosorbent assay technology. Rabbit anti mouse antibody was pre coated on a high affinity microplate. PGE2 specific monoclonal antibody was added to the wells, incubated, and bound to the solid-phase antibody. After washing, biotin labeled PGE2 and unlabeled PGE2 or samples were added to compete for binding to the limited binding sites on the monoclonal antibody. After washing to remove unbound material, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, the chromogenic substrate was added, and the depth of color reaction was inversely proportional to the concentration of PGE2. The reaction was stopped by adding a stop solution, and the absorbance value was measured at a wavelength of 450nm (reference wavelength 570-630nm). This kit has validated human, mouse and rat serum samples, but PGE2 of other mammals is also expected to be detected.

Detection Type: Solid phase competition method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, PGE2 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.08pg/ml

Detection Range: 15.63-2000 pg/ml

Recovery Range: 92-102%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Prostaglandin E2 (PGE2), also known as dinoprostone, is a natural prostaglandin that can be used as a drug. PGE2 is a drug of the oxytocin family. It binds and activates prostaglandin E2 receptors, which in turn leads to the opening and softening of the cervix and the expansion of blood vessels. It is a direct vasodilator that relaxes smooth muscle and inhibits the release of norepinephrine from sympathetic nerve terminals. PGE2 also plays a role in hippocampal synaptic plasticity and febrile response. In addition, pge2  It also stimulates tumor cell proliferation and differentiation and tumor associated neovascularization. PGE2 also inhibits T cell receptor signaling and may play a role in the resolution of inflammation.