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Description
Human CLEC7A ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against C-Type Lectin Domain Family 7, Member A (CLEC7A). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of C-Type Lectin Domain Family 7, Member A (CLEC7A) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human C-Type Lectin Domain Family 7, Member A ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Dectin-1, also known as C-type lectin domain family 7 member A, is encoded by the CLEC7A gene. It is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. It is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain that harbors partial immunoreceptor tyrosine-based activation motifs. It functions as a pattern recognition receptor for various β-1,3-linked and β-1,6-linked glucans from fungi and plants, thereby playing a role in the innate immune response. It is expressed on bone marrow dendritic cells, monocytes, macrophages, and B cells. Alternative transcript variants of its different isoforms have been identified. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Comfortable. Great Value for money. Easy to assemble. Great customer service.
Color: Black, Size: Pack of 1, Color: Black, Size: Pack of 1
I've been looking for a new chair to replace a cheap $30 chair i bought off of Amazon last year that had been killing my back and shoulders. I wanted something in the $150-$200 range and settled on the M18. Shipping was a fast 3 days with Prime and the box arrived in great condition.
All parts were separately wrapped or in their own boxes. Came with all hardware and nothing was missing. Assembly took me a little longer then normal because i have rotator cuff injury in my left shoulder so that arm is mostly useless. The most difficult part for me was attaching the wheels to the base. Other then that it was simple.
Once the chair was assembled i sat in it for the first time and started making my adjustments with the recline feature, armrest level along with the headrest. Next i reached around for the lumbar support knob and tried to adjust it but with my bad shoulder i decided to just get out of the seat and turn it around and adjust it with both hands. After spinning the chair a round to get at the lumbar knob i noticed a large crack in the plastic in the middle of the back of the lumbar plastic piece. I had not made any lumbar adjustments yet so i assumed this is how i received it. The lumbar support comes preinstalled to the backrest so it either was damaged before or during the shipping process. Either way it was damaged before being delivered to me. The lumbar support still functioned properly but it was still broken.
I decided to contact Sihoo's customer support that came along with the installation instructions regarding the damaged lumbar piece. I received a reply the next day and after sending them some more info about my order and a couple pics they shipped me a replacement part which took a week to arrive. I swapped out the damaged part and after owning the chair for a week so far i am really happy with my purchase.
Im about 5'10 180lbs. At its highest height level my feet reach the ground comfortably flat footed. The backrest fully covers my back and the headrest adjusts very well to sit on the back of my lower head/upper neck. The armrests only go up and down....not forward and back or tilt inwards. This is fine for me as they are grooved to fit your forearm while sitting at your desk/keyboard. The armrests are also a soft cushioned rubber material...not a hard plastic. The mesh of the backrest and headrest are good material and seem well made. The seat is very wide and the cushioning is firm which i prefer. Usually more firm cushioning seems to last longer and hold its form. The base of the chair is VERY sturdy and made of all metal. My chair is on carpet and holds its position very well. As far as recline goes you can set it to recline all the way back, half way or lock the chair in the upright position. You can also adjust the resistance on the reclining feature.
So that being said im very happy with my purchase. It arrived with a slight flaw which the company corrected in a fast manor and was very polite in their customer support. I personally don't have a lot of experience with many office chairs and have never used a high end chair($500-$1000+) chair before to make any comparisons. All my chairs for the most part have been from local stores like Staples or Office Depot. Id recommend this over anything they carry in this price range....and i went and looked at their products before buying this chair as well.
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Reviewed in the United States on September 1, 2023
★★★★★ 5
SIHOO M18 chair is amazing
Color: Black, Size: Pack of 1
I purchased the SIHOO M18 Ergonomic Chair a few years ago and have really enjoyed it. It’s very comfortable and offers multiple adjustments, whether you want to recline and relax or sit upright for work. The back support is also excellent, and I haven’t had any issues with the chair at all. You can easily tell it’s high quality from the build and appearance—no creaks or noises whatsoever. I highly recommend this chair to anyone in the market for a new office chair.
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Reviewed in the United States on June 12, 2026
★★★★★ 4
Good budget-friendly option
Color: Black, Size: Pack of 1, Color: Black, Size: Pack of 1
The chair arrived 4 days early and was easy to put together by following the instructions carefully. It feels sturdy and comfortable. It does support the lumbar region of the back and the arm rests are adjustable vertically as well as the head rest. I wish the arm rests were able to rotate in and out for added customization and that the back of the chair could be adjusted up and down for more precise support. But for a budget chair, it does a good job.
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Reviewed in the United States on June 4, 2026
★★★★★ 5
A solid chair for the budget conscious sitter.
Color: Black, Size: Pack of 1
While this is not THE most comfortable chair, let's face it - if you sit for long periods, what chair IS? I bought this chair because I'm making a career change. I've returned to school and spend hours at my desk. At the age of 50, sitting for a long time with my nose pointed toward a book can be hard on the seat, back and neck. I looked at all kinds of office chairs and settled on this one, primarily because it was on sale, was in my budget and so I clicked the "Add to cart" button and hoped for the best.
It was delivered, and I had to work that evening, and when I got home my 17-year-old son had assembled it. My theory on the simplicity of assembly can be deduced from that sentence above. He didn't have any extra parts left over and the chair was complete!
I sat down. I leaned back. I shifted the lever that keeps the chair in an upright position and sat straight up.
Okay! I can live with this. It has been almost two full months of using this chair and I have no problems.
Pros:
easy to assemble
sufficient comfort level - adequate cushion in the seat
can be leaned back or pop the lever in to stay straight up
rollers on bottom
headrest is adjustable
affordable
easy to clean
arm-rests can be moved up or down
height adjustment is self-explanatory - you'll figure it out
the "lumbar" support is actually comfortable - it's "there" if you back up to it but if you scoot forward just a bit, you won't feel it if you don't want to sit like that
Overall this seems like a chair that's going to last me for a few years, at least, and not fall apart. In the context of size, I'm not a small or extra larger person. I'm an average middle-aged woman with meat on the bones. The seat is plenty big enough.
I would recommend this chair as an upgrade to the hand-me-down office chair that just isn't comfortable any longer.
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Reviewed in the United States on April 1, 2023
★★★★★ 5
Love this chair!
Color: Black, Size: Pack of 1
I had an office chair that cost $300 more than this chair - had it for years, then things started breaking down on it. I read about this chair and, wow! I wish I had known about it sooner. It's more comfortable and it's not a chore to sit for a couple of hours working on designs. Wonderful design - supports my back well and it's very stable, easy to adjust.
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Reviewed in the United States on May 3, 2026
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