Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 13 - Aug 18
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human SLC39A4 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against zinc transporter ZIP4 (SLC39A4). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of zinc transporter ZIP4 (SLC39A4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Zinc transporter ZIP4 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Solute carrier family 39, member 4 (SLC39A4), also known as zinc transporter ZIP4 (ZIP4), is encoded by the SLC39A4 gene. It is a transmembrane protein that has been associated with acrodermatitis enteropathica. As a zinc transporter, it plays an important role in cellular zinc homeostasis. This gene encodes a member of the zinc/iron regulated transporter-like protein (ZIP) family. The encoded protein is localized to the cell membrane and is required for intestinal zinc uptake. Mutations in this gene cause acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been identified for this gene. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 20 reviews
Sort
Product Reviews
★★★★★ 3
Hydrates, leaves a slight white film, not travel friendly
Color: White, Size: 0.15 Ounce (Pack of 1)
I wear lip balm regularly. As a matter of fact, I always have some with me. Tubes are placed in various areas around my house, I have one in my car, in my backpack and a couple tubes at work. I'm seriously never without it so I kind of consider myself a lip balm expert. The only thing most of the ones I use don't have is SPF. Since I live in an area that is fairly sunny year round, you think I'd be better about that. Well, I'm looking to change that.
I saw this Sun Bum SPF 30 Mineral Sunscreen Lip Balm and read some of the reviews. They seemed overwhelmingly positive. I'm familiar with the brand and since it was covered under my HRA, decided to try this one out along with a few others. It glides on easily and moisturizes well, but it left a slight white film on my lips. Not my favorite but it did fade. It's unscented and doesn't have any flavor, which is good. I loathe lip balms with any type of taste to them. Good or bad. It has clean ingredients and offers great sun protection but it doesn't withstand heat itself. If you like to carry one in your pocket, backpack or keep in your car, it will melt.
I do like this lip balm but it has some negatives, as stated above. It made my lips feel soft and hydrated but it's not travel friendly. I wouldn't discourage buying it, but if you want a balm to carry around with you, I'd be hesitant to recommend it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 26, 2024
★★★★★ 5
Really works and it's safe.
Color: White, Size: 0.15 Ounce (Pack of 1)
I am a flats fisherman – this stuff really works, it's smooth, flavorless, reef-safe, and safe to lick. Reapply as often as you remember to.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 22, 2026
★★★★★ 5
Highly recommend. Any graininess and whiteness are minimal and insignificant.
Color: Blanco, Size: 0.15 Ounce (Pack of 2)
This is the best mineral(non-chemical) lip sunscreen I have found. Highly recommend. The first few times I used it, it felt ever so slightly grainy. Maybe the graininess rose to the top. Even if it was grainy all the way through, I would still recommend. There is a slight whitish cast to your lips-not noticeable or significant in my opinion. There is a very minimal flavor and it has a nice feel on your lips.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 7, 2026
★★★★★ 5
A must have for your summer bag
I am 100% devoted to Blue Lizard products! I got some extra sun and needed relief, fast. This non-greasy nor slimy cream is exactly what I didn’t know I was looking for. I had applied aloe alone to my skin and received no relief. Within minutes if not seconds of applying this, I had noticed improvement. The consistency is not too thick or too thin, it absorbs into the skin nicely and can be reapplied without getting cakey. It gave my sunburn relief while also moisturizing the skin. I haven’t peeled at ALL. I love that it has clean ingredients and a neutral fragrance. My skin is so sensitive, so having natural ingredients is a plus. Blue Lizard After Sun is now my holy grail and I will recommend it over and over.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 8, 2026
★★★★★ 5
HANDS. DOWN. AMAZING.
I have used other products for after sunning in the past. This stuff is AMAZING and I wish I could be a spokesperson for it. I am extremely fair skinned and used this on my vacation at the beach. I used some spf which always leaves me slightly red. I put this on every night and the redness was gone by morning! I was ready to hit the sun again! It doesn’t have a scent and doesn’t leave a sticky lingering feeling or weird smell either. Just moisturizing and safe for my acne prone skin. I will buy this and use this forever!!!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 5, 2026
recommand products
Oregon's Wild Harvest Alfalfa Capsules – USDA Certified Organic Herbal Supplements, 90 Count
49.90
Klaire Labs SFI Health Melatonin-SR 2 mg Capsules - Sleep Support (60 ct)
49.98
Wild & Organic Uric Acid Cleanse Drops – Tart Cherry, Turmeric & Chanca Piedra | Vegan, Sugar & Alcohol-Free 2 oz
38.98
Wellah So Long Soreness BCAA Powder (Frozen Lemonade) - 30 Servings with Hydration Complex
45.98
Bluebonnet Magnesium Potassium & Bromelain – Muscle Health Support | Non-GMO, Vegan, Gluten-Free
41.20