Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 22 - Jul 27
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human TSC22 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transforming Growth Factor Beta Stimulated Protein Clone 22 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | TSC22 domain family protein 1, also known as transforming growth factor-β-stimulated protein 22 (TSC22), is a protein encoded by the TSC22D1 gene. TSC22 encodes a transcription factor that belongs to a large family of early response genes. TSC22D1 forms homodimers through its conserved leucine zipper structure and heterodimerizes with TSC22D4. TSC22D1 exhibits transcriptional repression activity. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 14 reviews
Sort
Product Reviews
★★★★★ 5
Printer
Set name: Sublimation Printer
Bought is a Christmas gift. Has not been turned off since. Lol
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 29, 2026
★★★★★ 1
NOt good... not great... horrible
Set name: Sublimation Printer
I’m thoroughly disappointed with the Brother Sublimation Printer and would not recommend it. Out of the box, the setup process was more complex than anticipated, with unclear instructions and frequent errors. The print quality was inconsistent, with colors often coming out dull or inaccurate, despite multiple attempts at calibration. I experienced frequent paper jams, which disrupted my workflow and wasted a lot of materials. The printer’s software is buggy and doesn’t integrate well with other design programs, making it frustrating to use. The connectivity options were unreliable, causing frequent disconnections and delays. Additionally, the printer is quite noisy during operation, which can be distracting and bothersome. The build quality feels flimsy and not as durable as I expected. The customer support experience was also poor, with slow response times and unhelpful solutions. I faced issues with ink usage, as the cartridges seemed to run out quickly, leading to higher operational costs. The sublimation prints didn’t have the sharp, vibrant quality I was hoping for, making my final products look less professional. The lack of clear troubleshooting guides added to the frustration. The printer’s design is bulky and takes up more space than I anticipated. Overall, this Brother Sublimation Printer has been a letdown in terms of performance, reliability, and ease of use. I regret investing in this product and would advise looking for alternatives.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 9, 2024
★★★★★ 4
Pretty good for the price.
Set name: Sublimation Printer
So far so good. I have been producing a fair amount of items to date. My only wish was that the colors were more vibrant with this printer and it's ink.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 27, 2025
★★★★★ 5
Inkjets have come a long way
Pattern Name: Printer, Style: ET-15000
Just a lot to like about this printer and nothing I don't like. Very capable for the price.
I do mostly black and white printing, rarely print photos. (This will supposedly do a credible job of photo printing too but I haven't tried it). If you want professional caliber photo printing, you probably want the Epson 8550 which is similar to this, a bit more expensive, but does 6 color printing. If you are doing more like every day business printing the 15000 might be a better choice because it's built more for speed.
The tank system means I can just replace the black because I use it a lot. The black ink tank is double size of the others and based on my experience so far, I think I'll easily get over a thousand pages on the standard-vivid setting which is very strong black but still relatively high speed printing. Given that the black ink bottles are under $30 I think the cost per page will be comparable to laser.
The booklet/pamphlet printing can be complicated to work out with layers of PDF software and the Epson printer driver. But once you work it through, it's super capable. I've printed a lot of 12 x 18 double-sided for booklets and the paper handling has been flawless. (It can duplex automatically for 8 1/2 x 11 but for other sizes you have to manually duplex, which you can do by printing back/front or even/odd.)
It's not as fast as a laser printer but it's pretty fast -- inkjets have improved so much.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2026
★★★★★ 5
Perfect Printer for My Small Business
Pattern Name: Printer, Style: ET-15000
I purchased the Epson ET-15000 for my crafting and apparel business and have been very pleased with its performance. The print quality is excellent, colors are vibrant, and the large ink tanks help save money over time. It handles larger print sizes with ease and has become an essential part of my workspace.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 3, 2026
recommand products
SANYO 610 339 1700 Compatible Module with Original Bulb
320112.50
SAMSUNG BP96-01472A Compatible Module with Original Bulb
234.67
10-19 Jaguar X351 XJ XJR Front Left Driver A Pillar Trim Panel Suede OEM
55.95
00-02 Jaguar S-Type X200 Engine Left Driver Side Valve Coil Cover XR85285 OEM
57.95
Vibrant Aguascalientes, Mexico - A Painting Of A Building On A Hill
20.55