Mouse IL-5 High Sensitivity ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti- mouse IL-5 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. IL-5 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-5 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IL-5 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.14 pg/ml

Detection Range: 1.56-100 pg/ml

Recovery Range: 104-107%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 5 (IL-5) is a Th2 cytokine member of hematopoietic family, which is composed of 115 amino acids. It is similar to other members of this cytokine family (IL-3 and GM-CSF)Different, the activated form of this glycoprotein is homodimer. IL-5 is secreted by Th2 cells and mast cells. Its biological activity on B cells and other types of cells shows that IL-5 has an important functional effect on the activity of Th2 cells. IL-5 stimulates B cell growth and increases immunoglobulin secretion by binding to its receptor. It is also a key factor in activating eosinophils. IL-5 has been associated with a variety of allergic diseases, including allergic rhinitis and asthma. A large increase in IL-5 was observed in circulating tissues and tracheal tissues, while sputum eosinophils decreased.