Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 7 - Jul 12
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human FS ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with follistatin (FS) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of follistatin (FS) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Follistatin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Follistatin (FS), also known as activin-binding protein, is encoded by the FS gene. Follistatin is an autocrine glycoprotein expressed in nearly all tissues of higher animals. It is produced by follicular stellate cells (FS) in the anterior pituitary gland. It is a member of the TGF-β superfamily. FS increases follistatin levels by increasing muscle mass in certain core muscle groups, thereby increasing life expectancy. The relationship between follistatin and polycystic ovary syndrome is also under investigation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.4 ★★★★★
Based on 1299 reviews
Sort
Product Reviews
★★★★★ 5
One of the greatest books I’ve ever read
Format: Audiobook
I wish I could have met this man. I began having OBE’s about 8 years before I read the journeys trilogy. When he talked about the out of body simulations my head nearly popped off. I’ve had a few of those and never found them described in detail anywhere else. This is the greatest book and most profound trilogy on personal spiritual exploration and out of body travel ever written. Period. Such a gem and so incredible to be experienced outside the traditional contexts of yoga and meditation. A true original work. Thank you Bob. These 3 books changed my life and made me finally enquire “what is under the hood” in an OBE. What transports the astral body or consciousness from one dimension to another?
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 6, 2023
★★★★★ 5
Brought me back
Format: Kindle
Brought me back to understanding a deep knowing that has been there since I was a young child but couldn't understand what it was.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 19, 2025
★★★★★ 5
This is a must-read for all those who are seeking answers.
Format: Paperback
If you can read only one of Robert Monroe's 3 books ("Journeys Out of the Body," "Far Journeys" and "Ultimate Journey"), "Ultimate Journey" is the one to read. Monroe's books chronicle his amazing learning experiences as his ability to travel out of the body develops and his capability to remember and to put these odysseys into words increases. Without laboring over material covered in the first two books, Monroe sums up his earlier adventures and then writes about his most recent and significant discoveries on this, his spiritual journey to understanding.
If you have ever wondered if there is a God, why the Bible says that God is Love and in another place, shows God to be a vengeful being who orders the killing of many people, "Ultimate Journey" and the lessons taught to Monroe in his out-of-body state will help to clear up these questions. To say that "Ultimate Journey" will give you a whole new perspective on life, death and what lies beyond is an understatement. This is a must-read for those who are seeking answers!
And, if at all possible, read all 3 books in order. "Far Journeys" is definately the most difficult to read, since much of it reflects Monroe's efforts to translate non-verbal communications from non-humans into English. But the information in that book is voluminous and well worth the effort to read it. Happy reading, fellow seekers!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 16, 2012
★★★★★ 5
YOU can actually LEARN to do this!
Format: Paperback
"Eavesdropping on Animals” by George Bumann really was a welcome break for me as I spend so much time outside in Nature. George is also amazing at being able to read Nature in a way I had never seen before. His insight via really listening & paying attention to everything around him, is so precise - he can literally know where animals are without seeing them. I got this book mainly because HE wrote it & I knew it would be interesting. The thing that surprised me was he actually teaches you how to do what he does (ie: know exactly where a wolf pack is traveling by hearing a certain coyote call & knowing the terrain). I started simply by really paying attention to all of the bird songs going on outside my window very early each morning & could finally hear ones that were repetitively there & was able to figure out what they meant by getting outside & sitting on my patio as I drank my tea. If you LOVE NATURE, you would so get into this book. It’s a great teaching guide, plus his stories are so cool! Entertaining & informative to read.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 30, 2025
★★★★★ 5
Will forever change your experiences in nature!
Format: Hardcover
I am an instructor in animal language in the wildlife ecology department at two leading universities and I can attest that George is an expert animal whisperer/linguist. I've had the pleasure of knowing him and hearing his stories, and this book is an outstanding gateway into understanding the behavior of animals. It will reveal a whole new world to you every time you step outside, whether you live in an urban area or next to a wilderness.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 2, 2025