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Description
Rat ER ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Estrogen Receptor (ER) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Estrogen Receptor (ER) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Estrogen Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Estrogen receptors (ERs) are a group of proteins found within cells. They are receptors activated by estrogen (17β-estradiol). There are two types of ERs: nuclear estrogen receptors (ERα and ERβ), which are members of the nuclear receptor family of intracellular receptors, and membrane estrogen receptors (mERs) (GPER (GPR30), ER-X, and Gq-mER), which are mostly G protein-coupled receptors. Once activated by estrogen, ER can translocate to the cell nucleus and bind to DNA to regulate the activity of various genes (i.e., it is a DNA-binding transcription factor). However, it also has other functions independent of DNA binding. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.3 ★★★★★
Based on 24 reviews
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Product Reviews
★★★★★ 5
Works well
Size: 3 Fl Oz (Pack of 2), Style: SPF 30
Good product
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 29, 2026
★★★★★ 5
Good sunblock. New bottle design with easy to read expiration date.
Size: 8 Fl Oz (Pack of 1), Style: SPF 30
When reviewing this product. It ask you to put a STAR for:
Sun protection
Water resistance
I put 5 stars because, how would i know? I don't use it to go into water and you'd need to be a scientist testing how good the sun protection is. I just assume it works.
But i've used banana boat sunblock in the past. And it's been fine. No sunburns. As long as you remember to re-apply it because it's not meant to last all day.
Pros:
- It's sunblock
- It works (from my previous experience)
- New bottle design.
They changed the style of the bottle. The new bottle has the date on it. The old bottles only had a serial number. In order to find out if your old bottles are expired you had to go to the banana boat website and see the instructions on how to read the serial # that way you can know the expiration date.
The new bottle is more simple, the date is laid out easy to read on the bottle.
- Long expiration date:
I bought this sunblock around april or may of 2020 and the bottle says it will expire January 01, 2022
- Nice slim bottle to fit into a small bag.
Cons:
- It's a small 8 FL OZ bottle. My old bottle is a 10 FL OZ. Not sure if they still sell the 10FL OZ.
- It feels a little heavy and thick on the skin. But after a while it's fine.
- If you rub your hands / fingers around your eye, especially if you're sweaty. You might get the sunblock into your eyes. That has happened to me on hot summer days. It will make you teary eyed and irritate your eyes for a long while.
- I hear this sunblock may stain clothes. So far that has not happened to me.
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Reviewed in the United States on May 24, 2020
★★★★★ 5
Ideal Sunscreen for Backpacking Adventures!
Size: 3 Fl Oz (Pack of 2), Style: SPF 30
I recently used Banana Boat Sport Ultra Sunscreen Lotion SPF 30 on a backpacking trip, and it was absolutely perfect for my needs. This sunscreen provided excellent protection and convenience, making it a standout choice for outdoor activities.
First off, the SPF 30 rating offers reliable protection against harmful UV rays, which is crucial for long hours spent in the sun. The lotion goes on smoothly and absorbs quickly without leaving a greasy residue, which is a huge plus when you’re on the move.
The size of the bottle is spot-on for backpacking. It’s compact and lightweight, fitting easily into my gear without taking up too much space. This makes it convenient to carry and apply whenever needed, ensuring that I stay protected without adding bulk to my pack.
The water-resistant formula held up well even during sweaty hikes and water crossings. I didn’t have to reapply excessively, and it stayed effective throughout the day. The lotion also has a pleasant, non-overpowering scent, which is a nice bonus.
In summary, Banana Boat Sport Ultra Sunscreen Lotion SPF 30 is an excellent choice for anyone heading into the great outdoors. Its effective sun protection, ideal size for backpacking, and water-resistant properties make it a must-have for any adventure. Highly recommended for keeping your skin safe and comfortable while exploring!
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Reviewed in the United States on September 7, 2024
★★★★★ 5
Good stuff
Size: 3 Fl Oz (Pack of 2), Style: SPF 30
Banana Boat still smells good and works good too
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Reviewed in the United States on May 20, 2026
★★★★★ 5
Great sunblock, moisturizer, non-sticky and smells great!!
I’ve used a lot of sunscreen in my life being a fair skinned redhead and this product keeps me moisturized and prevented me from getting burned. We recently went to the French Polynesian islands and the sun hits hard down there and I never burned during our trip. I also absolutely loved how my skin felt moisturized and I never had dry skin from being in the pools and in the ocean. Plus, it smells amazing and you don’t feel sticky once it’s on.😊. Would highly recommend. Wish it was a little less in cost, but I love it.
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Reviewed in the United States on October 3, 2025