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Description
Rat ERRγ ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Estrogen-related receptor gamma (ERRγ) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the Estrogen-related receptor gamma (ERRγ) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Estrogen-related receptor gamma ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Estrogen-related receptor gamma (ERR-γ), also known as NR3B3 (nuclear receptor subfamily 3, group B, member 3), is a nuclear receptor encoded by the ESRRG (EStrogen Related Receptor Gamma) gene. This protein is a member of the nuclear hormone receptor family of steroid hormone receptors. This orphan receptor has no known physiological activating ligands, but 4-hydroxytamoxifen and diethylstilbestrol act as inverse agonists, inactivating ESRRG. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.2 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Saved Me From Buying A New Monitor
Style: 7-in-2 4K@30Hz
The MacBook Pro I use does not have an HDMI port for an external monitor. I had been using another hub that included an HDMI port which worked fine, but after 4 years it started wearing out. I didn’t immediately know this was a problem. My monitor had been flickering for some time, and eventually stopped working altogether. After much troubleshooting with inconsistent conclusions, I made the decision to try this hub. It works beautifully. My monitor started right up when I plugged it in. The hub fits right into the two USB-C ports on the left side of the laptop, and matches the computer. It makes a snug fit, so feels very secure. It includes all the ports I need. With 2 USB-C ports, I didn’t lose the two that are used to receive the hub. I like the fact that it feels like a part of the laptop and doesn’t connect like a dongle. Really happy with the product, and I didn’t have to replace my monitor.
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Reviewed in the United States on October 29, 2023
★★★★★ 5
Great little hub for MacBook Air!
Style: 7-in-2 4K@30Hz
I reviewed many before buying this one, and it suits my needs perfectly!
I run my MacBook Air in clamshell mode, lid closed most of the time driving a 43” 4K tv as my my main monitor when used at home, and the hdmi port supports 4K so you can happily run in 3840x2160 mode. Just make sure you get an hdmi cable that has the bandwidth to support 4K I.e. hdmi 2.1!
The dock fits closely to side of MBA and plugs into both usb-c ports so you have full power delivery.
It feels a high quality design, nicely engineered and takes up so little room in a MacBook slipcase!
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Reviewed in the United States on July 3, 2024
★★★★★ 5
Highly recommend.
Style: 7-in-2 4K@30Hz
The best travel hub I’ve ever received. Hardy and works incredibly well when I’m on the go literally has downsized the number of charging bricks and other peripherals/dongle. I need to carry with me. It makes it very easy to use the TV in your hotel as a second monitor.
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Reviewed in the United States on September 9, 2025
★★★★★ 5
Great!
Style: 7-in-2 4K@30Hz
I love that this thing adds extra ports to a very limited MacBook without the dangling terrible looking cord. The slim profile is a plus too. Additionally same color for aesthetics. Looks and performs great.
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Reviewed in the United States on June 9, 2026
★★★★★ 5
Great 10gbps USB-C Hub, worked with Anker support through compat issues with PD4 iteration 1
Original Review (updates below):
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The moment I noticed this thing on Amazon, I bought it and it literally just arrived. Finally, 4K60 over just USB-C with other ports (including making up to 10Gbps available to them) for a reasonable price. Unfortunately, the first two things I've done with it were both disappointing. I'll cut to the chase, the power delivery pass through isn't working with one of their own chargers.
The first device I tried connecting this with is my Anker PD4 with one USB-C cable as the only thing plugged into it so I can test the truly up to 100W (-15 for its own power) claims. I've tried connecting it with Apple's 2m USB-C charge cable (100w capable) and an equivalent CableMatters cable that also supports 100W. When plugged directly into my laptop, both of these cables immediately start charging it and show up as 100W power sources. When this Anker hub is plugged in and either of them is connected to the power delivery port on the side of it, the laptop does not charge (laptop is a 16" Macbook Pro, I've tried multiple ports just for the heck of it).
My second complaint is that when absolutely nothing is plugged into it, it becomes fairly warm to the touch. Why, when it's doing nothing, is it hot? I'm clearly not running data or even power through it so I have low hopes for it under load.
The fact that it doesn't work in exactly the scenario they want it to most (a MacBook Pro with one of their own chargers) seems like a pretty big fail. I've got lots of USB-C things though so I thought I'd grab a couple and try those. Using an Apple 96W USB-C charger, the device does pass through power delivery properly. It shows up as providing 79W to the laptop (siphoning off 17W in this instance). I also have a 56W Aukey charger that splits power between a USB-A port and a USB-C port that can provide 45W of power delivery. When plugged into that charger it does appear to work as well and appears in macOS as a 30W power source.
Maybe the problem is the Anker PD4? But it works fine providing power to the laptop directly. Would love to hear from Anker about these two products working together and what I should actually expect. Having it be unreliable for power delivery isn't great, but power and heat aside (it is getting a little bit warmer now that it's providing power from the Apple power adapter, but still definitely holdable), the hardware looks and feels good and the cable feels durable. Worth also noting that there's a white LED ring on the side of it that appears to be always on when it's plugged in. Don't know why it needs an LED.
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Update, June 22, 2020:
I've done some more testing and Anker has reached out to me to investigate what might be the issue. I can say that the hub works as I would expect from a quick test with my iPad Pro 11" with the above functioning chargers. It was able to pass through power with the 96W Apple Charger on the other end to both the iPad port as well as a Magic Keyboard port. The ethernet adapter showed up in iOS settings and an external drive was functional. When connected via the Magic Keyboard, which is a power only connection, as expected it provided only power.
Still no power passthrough of any kind when connected to the PD4.
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Update July 1, 2020:
I've written back and forth with Anker and they've sent me a replacement hub. It also doesn't work with the PD4. I've tried multiple USB-C cables but they have also shipped a cable to test with it. For now I'm increasing the review from 2 to 3 stars because I am more and more suspicious about the PD4 and less about this hub. They claim that they have tested the setup I have separately without issue, and I've sent video showing the problems I've encountered, so the troubleshooting is ongoing.
Have had a chance to use Ethernet on it without issue. Still need to try to test out its throughput when trying to use a 10Gbps USB-C enclosure and a 4K60 monitor at the same time (+ ethernet).
More updates to come.
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Update July 10, 2020:
I've continued to talk with Anker support and they shipped me a replacement PD4. The one I had was labeled as "Iteration 1" on its barcode, the one I got back was labeled "Iteration 2". I tested it out and it works perfectly with this hub, passing through the correct amount of power to multiple Macbook Pros and fast charging an iPad Pro. Clearly the fault doesn't lie with this hub and is instead an issue with the first iteration PD4, so I'm updating my review accordingly. Working with Anker support was great.
I've also been able to test USB-C throughput (without driving a display as well so far) and it properly utilizes 10Gbps. I've attached some extra images showing various devices connected to it (keyboard dongle, SD card, USB-C 10Gbps external SSD enclosure, USB sound card, ethernet).
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Reviewed in the United States on June 19, 2020