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Description
Human SCD ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Stearoyl Coenzyme A Desaturase (SCD). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Stearoyl Coenzyme A Desaturase (SCD) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Stearoyl Coenzyme A Desaturase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Stearoyl-CoA desaturase is an endoplasmic reticulum enzyme that catalyzes the rate-limiting step in the formation of monounsaturated fatty acids (MUFAs), specifically oleic acid and palmitoleic acid from stearoyl-CoA and palmitoyl-CoA. This enzyme is encoded by the SCD gene. Stearoyl-CoA desaturase-1 is a key enzyme in fatty acid metabolism. It is responsible for forming a double bond in stearoyl-CoA. This is how the monounsaturated fatty acid oleic acid is generated from the saturated fatty acid stearic acid. A series of redox reactions, during which two electrons flow from NADH to the flavochrome b5, and then to the electron acceptor cytochrome b5 and molecular oxygen, introduces a single-double bond in a series of methylene fatty acyl-CoA substrates. The enzyme complex adds a single-double bond between C9 and C10 of the long-chain acyl-CoA, de novo synthesis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
Made in America nano-hydroxyapatite Toothpaste that's Well Worth Buying.
Size: 4 Ounce (Pack of 3), Set name: E. Orange Cream, Watermelon Mint, Strawberry Mango
This review is pretty much the same as the one I wrote for the Boka Coco Ginger.
Boka Toothpaste is just that good.
I've been using the Japanese brand Apagard Premio toothpaste for a couple of years now and recently I noticed this Boka toothpaste while looking for alternatives that wold be less costly. I've been using this toothpaste since the end of May, 2025. I wish they would sell it in larger tubes.
Boka Toothpaste is very mild and makes brushing your teeth better. It doesn't have that harsh mint flavor which with most toothpastes feels like it's burning your mouth. It comes in multiple flavors and when you're done brushing your teeth, there is no need to rinse your mouth as it is a safe toothpaste to use.
After having used it for a few days, I decided that I will keep using the Boka brand from now on.
I just wish that most stores would carry this toothpaste instead pf selling all those major brands which are full of fluoride.
Did a bit of research and found that Boka Toothpaste has the same percentage of nano-hydroxyapatite as Apagard Premio which is 10%. So that means that Boka Toothpaste is just as good if not better.
No Fluoride
No Harsh burning Mint Flavor
Safe to shallow
Multiple flavors
10% nano-hydroxyapatite
Keeps your teeth Strong
And one thing I've noticed is nano-hydroxyapatite makes your teeth less sensitive by rebuilding your tooth enamel
I ordered four flavors, Watermelon Mint, Strawberry Mango, Orange Cream and Coco Ginger.
So far, I've tried the Coco Ginger and I was surprised at how mild it was and it didn't have the sharp taste that ginger usually has. It is good and I enjoy it. I also tried the Orange Cream which was good. I'm looking forward to trying the other flavors. The Orange Cream I'm sure my wife will like if I can get her to try it. But she can be very stubborn at times.
I see there are some other flavors which I intend to try such as the Cinnamint, Cucumber Dill, Lemon Lavender and Strawberry Basil. Not sure if I should try the Ela Mint as I don't know how strong or mild the mint will be.
I'm sure that if places such as Walmart brick & mortar stores sold this toothpaste, that it would sell really well.
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Reviewed in the United States on June 24, 2025
★★★★★ 5
Favorite toddler toothpaste
Size: 4 Ounce (Pack of 1), Set name: A. Strawberry Mango
I bought this for my 18month old who has all his teeth already. It works great. The smell is pleasant, the taste isn’t harsh, and the texture isn’t as thick as most toothpastes - perfect for a toddler. I’ve been using this for a couple of months now and have not noticed any irritation. He enjoys it about as much as any 18 month will, ha! It’s just as effective as fluorinated toothpaste. You get what you pay for. It’s a big tube!
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Reviewed in the United States on May 18, 2026
★★★★★ 4
Good flavor, not as full of tubes as I expected.
Size: 4 Ounce (Pack of 2), Set name: G. Orange Cream, Ela Mint
My kids have LOVED brushing their teeth with this. They had been fighting to let us brush for a while, so it was nice to find something they liked. My husband and I have been using the mint flavor, and it’s been good as well. We like the ingredients, and the texture is nice. The tubes both felt like they could have fit more, so I took off a star for size/value.
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Reviewed in the United States on March 9, 2026
★★★★★ 5
Very effective!
Size: 4 Ounce (Pack of 1), Set name: A. Strawberry Mango
I switched my son to this toothpaste and it has been great! I haven’t noticed whitening, but his teeth do look good and clean after he has brushed. He loves the flavor. And he is one to complain about “spicy” toothpaste. This toothpaste has never been whined about in my household. In fact, switching to this toothpaste has made brushing his teeth simpler because he enjoys the flavor so much. I have noticed it doesn’t foam up like other toothpaste’s do.
Another side story, and probably the biggest thing, I think this is actually shrinking my sons cavities. His pediatric dentist told me he would need fillings before his teeth could fall out at 12 (12 year molars). I saw the cavities on the xrays. A year later, the cavities are smaller. And the dentist said if things can maintain this way, there will be no need for fillings! He does brush morning and night, and often after lunch because we homeschool and he has that ability. But I’m just thrilled that my son could potentially avoid this procedure!
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Reviewed in the United States on April 23, 2026
★★★★★ 5
Love this stuff! Both toddler and mom approved
Size: 4 Ounce (Pack of 1), Set name: A. Strawberry Mango
I'm a repeat buyer! My toddler loves the taste and I love the ingredients. Boka is an awesome brand and I feel safe giving it to my kids and knowing their teeth are being cleaned.
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Reviewed in the United States on April 18, 2026