Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 17 - Aug 22
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human Resistin ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a resistin capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the resistin content in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Resistin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secretory cysteine-rich protein (XCP1), is a cysteine-rich peptide hormone derived from adipose tissue and encoded by the RETN gene. The human resistin propeptide is 108 amino acid residues long and has a molecular weight of 12.5 kDa. Resistin is an adipose-derived hormone (similar to a cytokine). Resistin increases LDL production in human liver cells and also degrades LDL receptors in the liver. Furthermore, resistin is produced and released from adipose tissue, serving endocrine functions that may be involved in insulin resistance. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 29 reviews
Sort
Product Reviews
★★★★★ 5
Excellent Rivals to Lovers!!
Format: Kindle
The tension and banter between Huntyr and Wold was delectable. I absolutely love the fallen angel and all of his flaws. Huntyr is amazing too being a badass FMC with some major trauma. The world building was great and I enjoyed the training aspect of the story. The writing was immersive and was in the story the whole time. The ending had quite a twist that I hadn’t anticipated and made my jaw DROP. Excellent job! I also loved the narration. Laura is one of my fave narrators!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 15, 2025
★★★★★ 5
Great start to the series! Loved it!
Format: Kindle
I really liked this story! It pulled me in from beginning to end and was engaging. I started to feel for the characters very early on, especially Effie and Julian. I loved how Julian knew right away that Effie was his mate and that meant for his whole bonded pack. For Effie, she didn't realize it meant that Julian was her mate but she felt at home with him, something she hasn't really felt very much at all. I love that we get so many details within this story as things do happen in a short span of time but it doesn't feel rushed or like things happen on fast forward due to that level of detail that we get. It doesn't get bogged down either. I love how Effie has that instant connection with all of her mates, she doesn't fully understand it but she feels it and is going with it. Her mates are very protective of her and she feels safe and at home with them, a feeling that she really has felt as long as she can remember, since she doesn't remember but for the last few years. I loved seeing her build her relationships with her mates as a whole and individually as well. I love how she doesn't just count anything out with any of her mates, even seeing the tension between some of them. She feels comfortable with them and is working on finding her place and becoming more confident in that with each other them. She also has lots of questions regarding the university and how she got there but she also wants to learn and figure things out. I really enjoyed this one and it ends on a slight cliffhanger and I definitely want and need the next one as I am fully engaged and hooked with the characters and want to see them find their happy ending.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 29, 2025
★★★★★ 4
Effie girl
Format: Kindle
I really enjoyed this intro into a new shifter world. This
is a university setting RH shifter book. We have 5 alpha males (4 wolves and 1 non wolf) all with their own problems but they all are instantly possessive and attentive towards our FMC Effie. This book takes place in the span of 3 days where a lot happens. I have a lot of thoughts. I really liked it yet I was also really frustrated at times with it. So here we go
Pros:
The 4 MC’s Tore, Dakota, Caedmon, and Julian are instantly possessive and all about Effie our FMC. I love this because lately all I’ve been seeing is the rejected mate story line and this is definitely NOT that. I love books where the alphas don’t have to be jerks and reject their women which ours don’t do.
The men our all relatable in trying to work through some of there own issues while still caring and being attentive to Effie. They aren’t perfect knights in shinning armor but it’s clear they try hard to make her feel special and important to them.
The writing is flawless and it was incredibly easy to continue reading (literally finished this book in one sitting)
Cons:
There was so much more inner monologue than actually dialogue between characters. Usually I don’t mine this but while reading it (on my kindle) the monologue had like 4 whole pages in between two sentences of dialogue here and there. It was just irritating not getting much actually conversation. (I was I think 12% in to this book before there was an actually dialogue. It was all Effies internal monologue or flash backs)
The world building left a bit to be desired because I still have tons of questions mainly about the types of wolves but I understand this is part of the naivety store line but still
This whole book takes place in the span of three days. No big ah ha action packed moments happened until the very last chapter. A lot of it was build up for the next 4 books I’m sure but it’s still frustrating because coming out of it I still feel like I know relatively nothing about this world. Each guy is a different species of wolf get we know nothing about any of them other than Fenrir wolves can’t shift.
- 4.5 stars because I definitely will pick up the second book. Book 1 ended in a cliff hanger so I’m eager to see what happens. I hope we get a bit longer storyline though and allow it time to develop so we get more information over all. This is a good book and I definitely recommend giving it a try. It’s a good start to a new series.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 6, 2021
★★★★★ 5
wow!!
Format: Kindle
I mean I was losing interest when I first started to read the first few chapters but I was still caught in the underlining description of what life was like for Effie. Then once she left to go to the University I was wrapped around the story. Very emotional and gripping as I understood the characters better. I'm glad I ordered the second book because I think this is going to be a very compelling series. I must say I'm not good with angst. But miss Sinclair has a talented flair for storytelling. I'm invested now to see it through.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 5, 2025
★★★★★ 5
Absolutely Fantastic!
Format: Kindle
Once again M. Sinclair has created another masterpiece!
This book was so unlike anything else I have read! It was such a unique twist on a shifter book that I was absolutely compelled from beginning to end. Effie made my heart all warm and fuzzy, she is just such a naturally kind person despite all of the things she has gone through. Her huge heart shines through in all she does.
Then their are her mates...protective, caring, and possessive Effie’s mates are just as much attracted and attached as Effie is to them. Their bond is instantaneous and strong. Despite Effie not knowing what to make of it at first and seeing it as to good to be true, her mates take it in totally stride.
I love the connections they have all built in such a short period of time. Effie brings a unique dynamic to this group. I am sitting at the edge of my seat waiting to see where this story goes. There are so many questions that that ending left and I genuinely cannot wait for more! I have grown super attached to these characters that more of their story can’t come soon enough!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 20, 2021
recommand products
Sennheiser TWISTED CABLE FOR IE 400/500 PRO In-Ear Headphones (Clear)
109.00
Gretsch Drums RN2-E8246-VP Renown 4-Piece Drum Kit (Vintage Pearl)
1534.99
Custom Gold White-Green Mesh Authentic Football Jersey
29.99
ISSAQUAH, I Love City's, White, Women's Short Sleeve Round Neck T-shirt 00012
20.00
Electro-Voice RE3-RE520-5L Wireless Handheld Mic System (FREQ: 5L)
674.50