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Description
Rat THBS1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 200 ng/mL). Then dilute to the following concentrations: 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 200 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 100 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a thrombospondin 1 (THBS1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of thrombospondin 1 (THBS1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Thrombospondin 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The thrombospondin (TSP) family is a group of structurally related secreted proteins widely distributed in the extracellular matrix of various tissues. It comprises two subfamilies: TSP-1/2 and TSP-3/4. TSP-1/2 has an inhibitory effect on angiogenesis. TSP-1 was first isolated from the platelet membrane by Baenziger et al. in 1971. It is secreted by normal fibroblasts, endothelial cells, smooth muscle cells, brain glial cells, and bladder epithelial cells. It binds to the membrane receptor CD36 and activates Fyn and Src tyrosine kinases, which are involved in endothelial cell proliferation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.6 ★★★★★
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Product Reviews
★★★★★ 5
10/10 sunscreen spray
I have combination skin that can get oily throughout the day, and I was excited to try the Naked Sundays SPF 50 Golden Glow Shimmer Setting Spray because I loved the idea of having sunscreen and a setting spray in one product.
The first thing I noticed was the packaging. The ombre gold to purple container looked so luxurious and intentional. The spray mist was fine and even, making it perfect to apply over my makeup with a smooth finish.
I love the scent. It smells fruity and delicious, which is such a refreshing change from the typical sunscreen smell that many other sunscreen sprays have.
Overall I loved this spray and it exceeded my expectations. It kept my makeup looking fresh all day long and gave my skin a beautiful, glowing finish. I would definitely recommend and I will 100% be purchasing it again!
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Reviewed in the United States on June 5, 2026
★★★★★ 5
Easy Spray Sunscreen with No Greasy Hands & No Harsh Smell
Really impressed with this sunscreen. The spray nozzle makes it incredibly easy to apply without getting my hands greasy, which is a huge plus. It feels lightweight on the skin and doesn’t leave a sticky or heavy residue.
One thing I loved is that it doesn’t have that typical strong sunscreen smell, it’s much more subtle and pleasant. I’ve also worn it during workouts, and it didn’t run into my eyes or cause any stinging when I was sweating, which is rare for me.
It layers well over makeup and gives a nice, subtle glow without looking greasy. Overall, it’s convenient, mess-free, and something I’ll definitely keep using for easy SPF reapplication throughout the day.
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Reviewed in the United States on April 29, 2026
★★★★★ 1
Nozzle doesn’t work on a $30 sunscreen!!
I’d give this zero stars if I could. While I imagine the glow is amazing, I wouldn’t know because the spray nozzle on this doesn’t work. Not a single spray. I’m assuming it’s clogged with glitter and I’ve tried everything unclog it but for $30, I shouldn’t need to decant or switch this into another bottle. I will end up doing that, so I can hopefully use it, but my complaint remains. It is also not eligible for returns or replacements. Cute but useless packaging.
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Reviewed in the United States on April 9, 2026
★★★★★ 5
My Favorite SPF Setting Spray
The Naked Sundays SPF 50 Golden Glow Shimmer Setting Spray gives the prettiest glowy finish while making SPF reapplication so easy. It feels lightweight, doesn’t mess up my makeup, and adds the perfect touch of radiance without looking too glittery. Definitely a staple in my routine!
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Reviewed in the United States on June 1, 2026
★★★★★ 5
Nice product
Has a nice smell and leaves a glow. Be careful of too many sprays as scent builds too. Glow is subtle and does not have a glitter look. I like it.
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Reviewed in the United States on May 13, 2026