SKU: 37795770240

Fixable Viability Dye 515

Sale price$94.50 Regular price$105.00
Save 10%

Pay in installments of $26.25 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Aug 15 - Aug 20

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Fixable Viability Dye 515Product Specification Stability & Storage 12 months from date of receipt, 20 C as supplied. Background Fixable Viability Dye 515 is excited by the Blue laser (with an excitation maximum of 488 nm) and has fluorescence emission maximum at 515 nm, Fixable Viability Dye 515 has similar emission to FITC. Fixable Viability Dye 515 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this

Product Specification


Stability & Storage

12 months from date of receipt, -20 °C as supplied.

Background

Fixable Viability Dye 515 is excited by the Blue laser (with an excitation maximum of 488 nm) and has fluorescence emission maximum at 515 nm, Fixable Viability Dye 515 has similar emission to FITC.

Fixable Viability Dye 515 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications.

Each lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.

Protocol

1.Wash cells one time in PBS buffer.

2.Add 1 µl of Fixable Viability Dye 515 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.

3.Resuspend cells at 1*10^7 cells/ml in Fixable Viability Dye 515, Incubate the mixture for 10-15 minutes at room temperature protected from light.

4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.

5.Resuspend the cells in Stain Buffer.

Note: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 37795770240

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.8 ★★★★★
Based on 28 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
P
Verified Purchase
Paulette Zastrow
Draper, US
★★★★★ 4
Authentic cry baby tears
Size: 1 Pound
I LOVE this candy and eat waaaay more than I should. My only minor gripe is that both times I've ordered from this company the bad has been slightly underweight- less than half an ounce off so not a huge deal, but for almost $13 a point I would rather it be spot on. It is the actual candy though and not a knock off so I am happy about that at least.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 19, 2026
J
Verified Purchase
joshua zeiger
Charlottesville, US
★★★★★ 5
Sour amazingness!!
Size: 1 Pound
Sugary sour goodness wrapped in a tasty candy amazing quality!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 13, 2026
M
Verified Purchase
Me
Massapequa, US
★★★★★ 5
Pretty good
Size: 1 Pound
So we're on the outside, not terribly sweet on the inside. Not the most sour candy I've ever had but very good and I really love sour things. It's fun watching people who don't like sour things eat them.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 28, 2025
K
Verified Purchase
Klara
New York, US
★★★★★ 5
Love it
Format: Hardcover
Love. Love. Love. I love this book. Every single word in this book is so precious to me. If Gesenius was like a strict father, this one is like a mother who so kindly elaborates on many difficult notions. Thanks a lot for such a great book ❤️ (Even if it were $1000, I would buy it! The bibliography is so kindly sorted out by a subcategory~♡)
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 5, 2026
R
Verified Purchase
Ready Writer
Port Orchard, US
★★★★★ 5
An indispensible Hebrew Language resource.
Format: Hardcover
This book is an amazing resource for anyone who has completed preliminary Hebrew such as with Pratico and Van Pelt in their Basics of Biblical Hebrew. This book goes above and beyond, it gives the diagnostic origin patterns for adjectives, patterns in noun formation as well as other components of speech, contrasts each of the seven major verb forms and the minor forms as well, and gives an excellent evolution of the utilization of the Hebrew language and its update in characters. It explains in its initial history a lot of unique statements in the Torah, and illustrations throughout give the reader an excellent idea about the Hebrew Scriptures. I bought this to aid in designing a language studies course at seminary and it has stood me in great help so far, going beyond anything I would have expected to get out of an intermediate level grammar and is an amazing, invaluable, and priceless resource, both to the professor, and the student. I recommend all people taking Hebrew purchase this book and add it to their collection and read it through cover to cover as well as using it as a topical reference guide.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 30, 2016

recommand products