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Description
F4/80 Recombinant Rabbit mAb (SDT-313-49)Product Specification Host Rabbit Antigen F4 80 Synonyms Adhesion G protein coupled receptor E1, EGF like module receptor 1, EMR1 hormone receptor, Gpf480, Adgre1 Immunogen Synthetic Peptide Location Cell membrane Accession Q61549 Clone Number SDT 313 49 Antibody Type Recombinant mAb Application WB, IHC P, ICC Reactivity Ms, Rt Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40%
Product Specification
| Host | Rabbit |
| Antigen | F4/80 |
| Synonyms | Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EMR1 hormone receptor, Gpf480, Adgre1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q61549 |
| Clone Number | SDT-313-49 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P, ICC |
| Reactivity | Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:500 | |
| ICC | 1:100 |
Background
The F4/80 monoclonal antibody has been used widely as a marker for mouse macrophages [PMID: 16087400]. EMR1-F4/80 is mainly present in the macrophage-containing stroma-vascular fraction. Furthermore, fibroblasts-like cells (adipoblasts), preadipocytes and adipocytes from the murine cell lines, 3T3-F442A and BFC-1, express CD14 and CD68 mRNA and protein as determined by fluorescence-activated cell sorter, but not F4/80 which, as expected, is strongly expressed in the macrophage cell line RAW264.7 [PMID: 16213494].
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