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Description
Mouse TRPV1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Transient Receptor Potential Cation Channel Subfamily V, Member 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transforming receptor potential cation channel subfamily V, member 1 (TRPV1), also known as capsaicin receptor and vanilloid receptor 1, is a protein encoded by the TRPV1 gene. It is the first member of the transient receptor potential vanilloid receptor protein group to be isolated, a subfamily of proteins. Its function is to detect and regulate body temperature. Additionally, TRPV1 provides the sensation of burns and pain (nociception). In primary afferent sensory neurons, it collaborates with TRPA1, a chemical irritant receptor, to mediate the detection of noxious environmental stimuli. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.5 ★★★★★
Based on 30 reviews
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Product Reviews
★★★★★ 5
Long lasting
Flavor Name: Bacon, Size: Medium
Great dog toy, long lasting
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 11, 2026
★★★★★ 4
Tough Chewer Approved but Not a Forever Toy
Flavor Name: Original, Size: X-Large
I do like this toy for what it is meant to do. It is definitely built for strong chewers and holds up way better than softer toys that get destroyed in minutes. The texture keeps dogs interested and gives them something to really work on, which helps with boredom and can support dental cleaning through chewing. It is one of those “finally something that lasts a bit” kind of toys for heavy chewers.
That said, it is not something you just toss in and forget about forever. Like most hard nylon toys, it will wear down over time and should be checked regularly for rough edges or changes in shape. Some dogs will also go through it faster depending on how intense their chewing style is, so replacement will eventually be needed. Girl, this is one of those “it survives the chaos better than most, but you still need to keep an eye on it” situations.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 5
The Remote Worker's Savior
Flavor Name: Bacon, Size: Medium
If you're like me, trying to balance deadlines and Zoom meetings while your furry co-worker incessantly nudges you for attention, then listen up because I've found the ultimate secret weapon: the Nylabone Chew Dog Toy. Trust me, it's a game-changer that will keep your dog busy and your productivity soaring.
Picture this: you're knee-deep in spreadsheets, buried in emails, and your dog is bouncing around like a furry tornado, demanding playtime. Enter the Nylabone Power Chew, the superhero of dog toys that saves the day. Its durability is off the charts, my friends. It can withstand a full day of canine chewing fury, leaving your furniture unscathed and your dog blissfully entertained.
But here's where the magic happens. This toy isn't just tough; it's also infused with a bacon flavor that sends your dog's taste buds into a frenzy. It's like having a bacon-scented office right in your home! As your dog sinks their teeth into this baconlicious wonder, their focus shifts from your keyboard to their newfound chewy delight. Suddenly, the tug-of-war with your important documents becomes a distant memory.
Now, let's talk about the beauty of this toy's distraction power. With the Nylabone Power Chew in play, your dog becomes engrossed in their chewy mission. They're occupied, happily gnawing away, while you conquer your work tasks like a true champion. It's like having a personal assistant who doesn't interrupt you every five minutes with a squeaky toy demand.
But don't be fooled by its functionality; the Nylabone Power Chew brings more than just peace and quiet. It brings laughter and joy to your work-from-home routine. Watching your pup go to town on this bacon-infused wonder is like having a built-in comedy show right in your home office. It's the perfect stress relief during those never-ending conference calls.
And let's not forget the Nylabone brand's commitment to quality. This chew toy is built to last, crafted with non-toxic materials that keep your beloved furry friend safe and sound. It's like having a reliable coworker who never lets you down.
So, my fellow remote workers, if you're desperate for a productivity boost and a furry coworker who won't pester you all day long, the Nylabone Chew Dog Toy is your secret weapon. It's a dog toy miracle that keeps your pup happily occupied while you conquer the work world. Say goodbye to interruptions and hello to a harmonious work-from-home environment. Trust me, you'll wonder how you ever survived without it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 25, 2023
★★★★★ 5
Clean teeth as well!!
Flavor Name: Bacon & Chicken, Size: Small
If your dog is a chewer or just bored, these bones are amazing. All of my dogs love and have loved Nylabones. I consider them a must to give your dog as an outlet to chew when bored or when in need of stimulation. Replace as necessary. I always have a pack on standby.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 31, 2026
★★★★★ 3
Not for chewers
Flavor Name: Bacon & Chicken, Size: Small
My dog liked these okay. He was able to chew little pieces off though.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 29, 2026
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