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Description
Mouse ADH ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an alcohol dehydrogenase (ADH) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of alcohol dehydrogenase (ADH) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Alcohol dehydrogenase（ADH) ELISA kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Alcohol dehydrogenase (ADH), abundant in animal liver, plant, and microbial cells, is a zinc-containing metalloenzyme with broad substrate specificity. It is an 80 kDa dimer comprising a group of isoenzymes that are capable of converting ethanol to acetaldehyde. In mammals, this is a redox reaction involving the coenzyme nicotinamide adenine dinucleotide (NAD+). ADH catalyzes the oxidation of primary and secondary alcohols to aldehydes and ketones and can also influence the reverse reaction. In mammals, ADH and aldehyde dehydrogenase (ALDH) form the ADH system, participating in ethanol metabolism and serving as crucial metabolic enzymes in animals. As a key enzyme in the metabolism of the primary short-chain alcohol, ADH plays a vital role in numerous physiological processes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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Product Reviews
★★★★★ 3
Almost perfection
Color: Khaki, Size: XX-Large
I have really liked this polo the fit is great material is so lightweight and breathable Chef's kiss. the one thing i didn't like is the white accent parts are like heat applied and peeled off after 2 washes it doesn't do anything to the polo but still a little disappointing i just peeled the rest of them off so quality leaves a little bit to be desired there. i wish they didn't include them and it would be 5/5 for me but if after 4 or 5 wears and 2 washes it peels off that is a little disappointing
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Reviewed in the United States on May 17, 2026
★★★★★ 5
This Shirt Is So Cozy My Husband Loves It — Perfect Fit and Style!
Color: Turquoise, Size: X-Large
I recently picked up the Alex Vando Men’s Golf Shirt, and I have to say, it has quickly become one of my husband’s favorites in his wardrobe. From the moment he tried it on, he raved about how soft and comfortable it feels. The material is lightweight and breathable, yet it doesn’t feel flimsy — it strikes the perfect balance for both everyday wear and active use.
The moisture-wicking and quick-dry properties are a game-changer. He’s worn it for golf, casual outings, and even on warmer days, and it keeps him cool and dry without sticking uncomfortably. The UPF30+ is an added bonus for sunny days outside, giving some extra peace of mind during golf or tennis sessions.
What I really love — and what he notices — is the attention to detail. The 3D checkered texture gives the shirt a subtle, sophisticated look that feels premium. The color-matched snap buttons are secure and easy to use, and the fine line detailing along the placket adds a touch of depth. The side panels and slit hem make movement easy, so he doesn’t feel restricted during activities. It’s clear this shirt is designed with both style and performance in mind.
Fit-wise, it’s fantastic. It flatters his physique without feeling tight, giving a clean, streamlined look. He’s worn other golf polos before that were either too stiff or too loose, but this one hits the sweet spot perfectly.
Another huge plus is the practicality. This shirt is wrinkle-resistant and washes beautifully, requiring no ironing. That makes it perfect for busy days when he wants to look sharp without extra effort.
Overall, the Alex Vando Men’s Golf Shirt is a winner. It’s stylish enough for business casual, functional enough for sports, and incredibly comfortable for everyday wear. My husband loves it, and I’ve already noticed him reaching for it again and again. If you’re looking for a high-quality, versatile polo that combines comfort, style, and performance, this is absolutely worth trying.
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Reviewed in the United States on March 11, 2026
★★★★★ 5
Good
Color: Teal, Size: XX-Large
"I highly recommend this purchase. The product's quality and performance have fully met my expectations."
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Reviewed in the United States on June 4, 2026
★★★★★ 5
Good quality
Color: Teal, Size: XX-Large
The shirt is just as advertised.
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Reviewed in the United States on May 19, 2026
★★★★★ 4
Good
Color: Teal, Size: XX-Large
Good
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Reviewed in the United States on May 21, 2026
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