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Description
CD45 Mouse mAb (429-1)Product Specification Host Mouse Antigen CD45 Synonyms Leukocyte common antigen (L CA) Immunogen Synthetic Peptide Location Cell membrane Accession P08575 Clone Number 429 1 Antibody Type Mouse mAb Isotype IgG1,k Application WB, IHC P, ICC, ICFCM Reactivity Hu Purification Protein G Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months
Product Specification
| Host | Mouse |
| Antigen | CD45 |
| Synonyms | Leukocyte common antigen (L-CA) |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P08575 |
| Clone Number | 429-1 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1250 | |
| IHC-P | 1:1000 | |
| ICC | 1:125 | |
| ICFCM | 1:500 |
Background
CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
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