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Description
Mouse STAT3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 100 ng/mL). Then dilute to the following concentrations: 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, 1.5625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 100ng/mL standard working solution into the first EP tube and mix thoroughly to make a 50ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Signal Transducer and Activator of Transcription 3 (STAT3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the amount of Signal Transducer and Activator of Transcription 3 (STAT3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Signal Transducer And Activator Of Transcription 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Signal transducer and activator of transcription 3, acute phase response (STAT3) is a transcription factor encoded by the STAT3 gene. It is a member of the STAT protein family. In response to cytokines and growth factors, STAT3 is phosphorylated by receptor-associated Janus kinases (JAKs), forming homo- or heterodimers and translocating to the nucleus, where it functions as a transcriptional activator. Specifically, STAT3 is activated following phosphorylation at tyrosine 705 in response to ligands such as interferon, epidermal growth factor (EGF), interleukin (IL-) 5, and IL-6. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 1.56-100 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 5
Sony STR-DH190 is an Amazing Deal. Don't pass it up.
Style: Stereo Receiver, Configuration: Receiver
I bought my STR-DH190 receiver to put in my "man cave" pool room. I respect SONY products, but I realized this 2-channel stereo receiver was a budget item, seemingly so inexpensive it must just be an entry-point-only receiver. I was shocked by its quality, sturdy construction, versatility and audiophile sound. It has Bluetooth connectivity, and a phono input for turntables. I hooked up a CD player, turntable, reel-to-reel, cassette deck, iPod, sub woofer and two sets of high end bookshelf speakers. The fronts are SONY Sscs5 3-Way 3-Driver Bookshelf Speakers. The rears are Polk. The sound is shocking, and everyone who hears it can't believe how it rocks. Now that this receiver is actually on sale at almost a "give-away" price, I'm tempted to buy another one as a backup in case one of my other systems needs a replacement receiver. It is Bluetooth ready, so I pair an Echo Dot with it for streaming. It is an amazingly versatile and reliable product. Highly Recommended.
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Reviewed in the United States on July 9, 2025
★★★★★ 4
Quite Good, but maybe lacking a few inputs, Optical Audio and AM radio
Style: Stereo Receiver, Configuration: Receiver
Very basic, but seems to be the usual good quality Sony product. I bought this when my decades-old SOny receiver finally bit the dust.
This receiver, at a decent price is a LOT more basic than my decades-old earlier receiver. That receiver had multiple inputs-many which I didn't use but were there for audiophiles all the same. Things like inputs for DAT, inputs for MD audio. analog videos in and out, Super video in and out, Component video, standard cassette deck, phono, component video (multiple), DVD in and out, component video, and more audio channel options I can't even remember. And as old as this receiver was it actually had Optical audio as well! I REALLY wanted to replace that old receiver with the exact same thing. albeit the modern model but that doesn't exist.
SO I bought this one.
Take a look and you'll see very few inputs, but oddly it had all the ones I actually needed for my old set-up-with NO extras though. I'm kind of shocked that this receiver doesn't even have the Optical audio which is kind of a standard now for modern TVs and I had to actually buy a cheap little 'converter box' which takes the optical cable and converts the digital audio into the standard RCA analog audio and I plug that into one of the available inputs in the receiver so that I can play my TV sound through the receiver/stereo system. I'm still kind of shocked that this receiver doesn't have that Optical, that would be the first thing i'd add to really make this a bargain and much more desirable.
So I had to have a receiver for my old cassette deck, phono, etc. and standard CD player as well as DVD/Blu Ray audio sound (as concert DVDs don't sound too good on the standard speakers which come with modern TVs. So i've used EVRY input in this new unit and I really couldn't have bought it if it had a single less input.
So in addition to having an input for Optical audio the other thing I wished it had was a receiver for AM radio, even if AM isn't too popular anymore.
So maybe the lack of the Optical audio, additional inputs for other sources and no AM radio is the reason why this receiver is so reasonably priced, but well, i'm happy with it all the same.
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Reviewed in the United States on April 27, 2026
★★★★★ 5
Great product
Style: Stereo Receiver, Configuration: Receiver
This is a great basic receiver. It's very easy to understand, setup and operate. This receiver sounds excellent and includes Bluetooth so I can stream Apple Music and Spotify to the receiver. My only negative comments would be that the speaker connections are the spring-loaded terminals which require thin gauge speaker wires or Flex Pin Banana Plugs. Also, you should be aware the FM Antenna (provided) is a wire antenna utilizing a strange plug-in adapter. It was perfectly adequate for what I needed however I think it could be an issue if you were in a remote area that required a mast antenna.
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Reviewed in the United States on April 9, 2026
★★★★★ 5
Shockingly Great: Do Not Be Skeptical Of The Ultra Low Price
Style: Stereo Receiver, Configuration: Receiver
Do not allow the extremely low price on this Sony STRDH 190 stereo receiver to in any way prejudice you against considering it as a wonderful entry-level stereo receiver. I have purchased thousands of products on Amazon over the past 25 years, and I cannot think of any item that pleasantly surprised me more profoundly than this one. I also purchased the matching (style) Sony turntable PS-LX310BT which works perfectly with the STRDH 190 receiver.
Sony's lowest priced receiver now sells for the astounding bargain price of only around $200, though I caught it on sale for closer to $150... and as impossible as this is the believe, it has in recent years gone on sale for as little as $99(!) I have no idea how Sony makes a profit at these price levels.
Now I know, most people would think, "there is just no way a $200 receiver is high quality"... but I am telling you, that is dead wrong in this case, and you should prepare to be blown away by what you get at this crazy low price point.
This is NOT a no frills unit. It is feature packed.
It has connections for TWO PAIR (four total) speakers, and PLENTY of clean no-distortion power to drive all four speakers to loud and clear volume levels. It also has an A/B speaker switch to play through speaker set A, or speaker set B, or all four speakers at once.
It has a phono stage pre-amp which is actually very good. Seriously, it is much better than the phono stage pre-amp built into my Emotiva integrated amp for which I paid $1,000. It plays vinyl loud with no distortion. You will NOT need an external phono pre-amp.
It has excellent Bluetooth wireless connectivity built in... no trouble getting it to instantly recognized sources and play them perfectly. You can play all the music on your iPhone or other digital device, wirelessly.
It has a huge allotment of extra connections, ... a whopping FOUR inputs... so you can also hook up a CD player, and another external device of your choice, and another one after that, and another one after that! (WOW). And that's is not counting your PHONO input which is actually a fifth input connection. Astounding accommodation of external sources at this (or almost any) price point.
If you are a dinosaur who listens to FM ... it's got an FM receiver with 30 station presets, and an output to hook up a proper external FM antenna, and a long-wire FM antenna is included in the package
Sony says the "HiRes" audio produced by this unit is "superior to CD quality"... though I am not sure how to measure that claim, especially if the extrnal source you are using is a CD player.
It comes with a very high quality remote control, with real buttons, and many features.
You can actually program each of your external sources to appear in the illuminated display as the specific names you want, rather than a generic default source name like "CD".
The low price may raise an eyebrow, and the fairly light weight (much heavier on one side than on the other curiously) nature of the unit may also cause doubt... but only until you set it up and start listening, and your jaw drops.
I would put this sub-$200 receiver up against any receiver on the market priced under $800, (and probably several of them priced at twice that much!) and I believe you will not be disappointed in the sound quality of Sony's entry-level unit. You certainly will NEVER find another receiver anywhere near this price point with so many features and inputs... all of which work flawlessly.
This is the greatest bargain in the world of affordably-priced HiFi equipment.
It is easily worth multiple times the asking price.
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Reviewed in the United States on May 18, 2025
★★★★★ 5
Overall Excellent - Thorough Review - READ ME
Style: Stereo Receiver, Configuration: Receiver
I'm not an audiophile per se, but I do have elevated listening preferences. I enjoy finding the value in devices, and system configs. So that's the reason I tried this more budget-friendly receiver.
Aesthetics: The look is very sleek and modern, which is great but sometimes I miss the nostalgia of more knobs. There is no base/treble knob and most everything is controlled by remote. That's fine I suppose, just be aware. The volume knob is very large and traditional which I love, but is also very stiff and not buttery smooth like you might expect. Perhaps that will break-in.
Sound Quality: Wow, this sounds incredible. There is definitely a DSP happening that is noticeably awesome. It's referred to by Sony as Pure Direct. If you want to by-pass this, you can, but you'll hear right away the difference is major. I'm using simple Klipsch bookshelf speakers, and my expectations are surpassed on how much they come to life with bright and rich sound. Definitely a nice pairing for those shopping for speakers along with this unit. Overall the SQ is gonna be tough to beat without spending exponentially more.
Remote: The remote is nice, sleek, and has what you need to quickly access. No complaints there.
Installation: Takes minutes, super easy. Didn't follow any instructions due to my general confidence hooking up home audio gear.
Overall: I highly recommend this one, and I'm picky. Initially, I fully expected to return this receiver, but plan to keep it. The only thing that may change my mind, is if I miss the nostalgia... then I'll have to go vintage. But no way a vintage receiver will sound this good. Props to Sony. Well done :)
A+
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Reviewed in the United States on January 18, 2024